胃癌组织中的环氧化酶-2的表达与幽门螺杆菌感染的关系  

Expression of COX-2 and its relationship with H. pylori infection in human gastric carcinoma

作　　者：李强[1] 肖炜明[1] 丁岩冰[1] 薛艳[1] 倪庆[1] 严志刚[1] 

机构地区：[1]江苏扬州市第一人民医院,江苏扬州225001

出　　处：《中国现代医学杂志》2008年第12期1688-1691,共4页China Journal of Modern Medicine

基　　金：江苏省卫生厅科研项目(Z2006027)

摘　　要：目的探讨环氧化酶-2(COX-2)在胃癌组织中的表达及与幽门螺杆菌(H.pylori)感染的关系。方法采用免疫组化EnVision两步法检测47例胃癌组织及相应的20例癌旁组织COX-2的表达;用Western blot方法检测10例胃癌组织和相应的癌旁组织中COX-2蛋白的表达;用快速尿素酶诊断法和13C呼气试验两种方法检测胃癌患者H.pylori感染状况。结果免疫组化检测胃癌组织中COX-2的阳性表达率明显高于癌旁组织(P<0.01);有H.pylori感染的胃癌组织中COX-2的阳性表达率较未感染的胃癌组织中高(P<0.01)。Western blot方法检测胃癌组织中COX-2的蛋白表达高于癌旁组织(P<0.05)。结论COX-2在胃癌组织中高表达,H.pylori感染可能上调胃癌组织中COX-2的表达。[Objective] To investigate the expression of COX-2 and its relationship with H. pylori infection in human gastric carcinoma. [Methods] Immunohistochemistry was performed to detect the expression of COX-2 in 47 cases of gastric cancer and 20 cases of cancer-adjacent tissues, while Western blot was used to examine the protein expression of COX-2 in 10 cases of gastric cancer and their corresponding cancer-adjacent tissues. H. pylori infection were detected in all the patients with gastric cancer by fast urea enzyme test and 13C breath test. [Results] Immunohistochemistry showed that the expression of COX-2 in gastric carcinoma was significantly higher than that in normal mucosa（P〈0.01）, and its expression in patients with H. pylori infection was higher than that without H. pylori infection （P〈0.01）. Western blot also showed that the protein expression of COX-2 in gastric carcinoma was significantly higher than that in normal mucosa （P〈0.05）. [ Conclusion] COX-2 is over-expressed in gastric carcinoma. H-pylori infection could up-regulate the expression of COX-2 in gastric cancer tissues.

关 键 词：胃癌 环氧合酶-2 幽门螺杆菌 

分 类 号：R735.2[医药卫生—肿瘤]