宫内蛋白营养不良对大鼠胰岛素抵抗的影响  

Malnutrition during pregnancy causes insulin resistance in rats

作　　者：刘晓梅 潘莉莉[2] 李书琴[1] 卢岩[1] 

机构地区：[1]中国医科大学附属盛京医院实验中心,辽宁沈阳110004 [2]中国医科大学附属盛京医院检验科,辽宁沈阳110004

出　　处：《中国现代医学杂志》2008年第12期1692-1695,共4页China Journal of Modern Medicine

基　　金：辽宁省自然科学基金资助项目(9910500709)

摘　　要：目的探讨宫内营养不良造成的宫内发育迟缓(IUGR)大鼠发育过程中胰岛素敏感性和糖代谢的变化。方法采用孕鼠低蛋白饮食法建立IUGR模型,测定雄性子鼠生后每周体重增长和3周、8周和12周的肾周脂肪重量,观察8周子鼠胰岛素耐量实验变化。结果UGR组子鼠平均出生体重明显低于对照组,4周时达到对照组平均水平,8周到12周IUGR组体重明显超过对照组。且8周和12周时,IUGR组的肾周脂肪重量明显高于对照组。IUGR子鼠3周时空腹血糖、胰岛素和游离脂肪酸水平与对照组差异无显著性,8周后I-UGR子鼠出现高胰岛素血症,胰岛素抵抗指数(IRI)增高。12周时IUGR组出现高血糖。8周和12周时,IUGR组子鼠血清游离脂肪酸水平明显高于对照组。胰岛素耐量实验显示IUGR子鼠对外源性胰岛素反应迟钝。结论宫内蛋白质营养不良诱导IUGR大鼠葡萄糖稳态发生永久改变,成年后出现2型糖尿病表现。[Objective] To explore the insulin sensitivity and glucose metabolism in rats of intrauterine growth retardation （IUGR） caused by malnutrition during pregnancy. [Methods] IUGR rat models were established by maternal protein-malnutrition. Male offsprings were studied. The body weights of rats since birth to 12 weeks of age were weighted. The weight of perinephric fat pads at W3, W8 and W12 were examined. At 8 weeks of age, rats were subjected to an insulin tolerance test to evaluate insulin sensitivity in IUGR animals. [Results] Birth weight of IU- GR animals were significantly lower than that of control groups until 4 weeks of age, when IUGR rats caught up to controls. Between 8-12 weeks, the growth of IUGR rats surpassed that of controls with perinephric fat pads significantly increased. No significant differences were observed in blood glucose and insulin levels at 3 week. However by ＇8 week IUGR rats developed hyperinsulinemia and high IRI. At age 12 weeks, IUGR rats had mild fasting hyperglycemia besides high IRI. Insulin tolerance test showed a significantly blunted glycemic response to exogenous insulin in IUGR rats. [Conclusions] These data suggest that malnutrition during pregnancy can induce permant changes in glucose homeotasis and lead to type 2 diabetes in adulthood.

关 键 词：宫内发育迟缓 大鼠 胰岛素抵抗 

分 类 号：R-332[医药卫生]