大黄鱼Piscidin-like抗菌肽基因部分cDNA的克隆与序列分析  

Cloning and Sequence Analysis of Piscidin-like Antimicrobial Peptide Partial cDNA Derived from Large Yellow Croaker,Pseudosciaena crocea

作　　者：陈勇[1] 苏永全[1] 吴常文[2] 王军[1] 毛勇[1] 徐斌[1] 

机构地区：[1]厦门大学海洋与环境学院 海洋生物重点实验室,福建厦门361005 [2]浙江海洋学院,浙江舟山316000

出　　处：《厦门大学学报（自然科学版）》2008年第4期575-579,共5页Journal of Xiamen University：Natural Science

基　　金：国家863计划(2006AA10A405);科技部国际科技合作项目(挪威)(2006DFA32960)资助项目

摘　　要：利用RT-PCR、3’RACE等技术，从养殖大黄鱼免疫器官头肾和脾组织克隆出Piscidin-like抗菌肽基因的cDNA（311bp），其中包含部分信号肽、完整的成熟肽与优势域的编码区、终止密码子以及3＇端非编码区（3’UTR）和典型的多腺苷酸化信号（AATAAA）．获得的cDNA编码80个氨基酸，与已报道的鱼类Piscidins家族抗菌肽序列比对，表明存在相似的结构域，相似性（Similarity）和一致性（Identity）分别为50N～77％和41％～70％，分子量为2596．06u，理论等电点为12．10，与该家族抗菌肽普遍特征相吻合，属于Piscidins家族的新成员．By using RT-PCR and 3＇RACE,a Piscidin-like antimicrobial peptide cDNA fragment from anterior kidney and spleen of large yellow croaker,Pseudosciaena crocea challenged by Vibrio harveyi was isolated. The amplified cDNA was 311 bp in length including coding region,3＇UTR and a canonical polyadenylation signal （AATAAA）, encoding 80 amino acids which composed partial signal peptide, entire mature peptide and prodomain. The deduced amino acid sequence showed 50 % -77 % similarity and 41% - 70% identity after BLAST with Other members of Piscidins family. The property of peptide with 2 596.06 u,isoelectric point of 12.10 significantly matched the typical characteristiscs of Piscidins. This pioneer research strongly indicated that Piscidin-like cDNA from large yellow croaker was a new member of the Piscidin gene family.

关 键 词：抗菌肽 Piscidin 大黄鱼 

分 类 号：Q71[生物学—分子生物学] P745[天文地球—海洋生物学]