COX-2抑制剂对胰腺癌细胞株CFPAC-1增殖及凋亡的影响  

Effects of COX-2 Inhibitor on the Growth and Apoptosis of Pancreatic Cancer Cells

作　　者：李蓉[1] 杜冀晖[1] 苏卓娃[1] 李佳璇[1] 蔡安季[1] 周蓓[1] 麦丽文[1] 

机构地区：[1]广东医学院附属深圳南山人民医院,深圳518052

出　　处：《热带医学杂志》2008年第6期550-552,共3页Journal of Tropical Medicine

基　　金：深圳南山科技局项目(No.2006011)

摘　　要：目的探讨选择性环氧合酶抑制剂Celebrex(西乐葆)对胰腺癌CFPAC-1细胞增殖、凋亡的影响及其作用机制。方法采用CCK-8比色法观察不同浓度、不同时间点的Celebrex对CFPAC-1细胞增殖的影响;流式细胞术检测不同浓度Celebrex作用下CFPAC-1细胞凋亡和细胞膜P-gp表达的变化。结果8、16、32、64μmol/L的Celebrex作用于CFPAC-1细胞后,可明显抑制胰腺癌细胞株CFPAC-1的增殖,并呈时间和剂量依赖性,与对照组比较,差异有统计学意义(P<0.05);同时细胞凋亡率显著上升,而细胞膜MDR1(P-gp)表达阳性率显著降低(P<0.05),上述作用均成剂量依赖性。结论Celebrex降低胰腺癌细胞株CFPAC-1细胞膜表面P-gp的表达,从而诱导细胞凋亡,这可能是其抑制胰腺癌细胞株CFPAC-1增殖的作用机制之一。Objective To examine the effect of Celebrex on the growth and apoptosis of CFPAC-1 pancreatic cancer cell. Method CFPAC-1 cells were treated with various concentrations （0,8,16,32,64 p.mol/L） of Celebrex, and the proliferation was measured by CCK-8. Apoptosis and expression of P-go was detected by flow cytometry. Result Celebrex （8-64 μmol/L） was found to time-and dose-dependently suppress the growth of CFPAC-1 cells, and the difference is significant （P〈0.05）. Celebrex was also found to significantly （P〈0.05） induce apoptosis and decrease the expression of multi-drug-resistance proteins （MDR1, P-go）in a dose dependent manner. Conclusion Reduction of the expression of P-go, inhibition of the proliferation, and induction of apoptosis in CFPAC-1 cells may be responsible for the anti-tumour effect of Celebrex.

关 键 词：COX-2抑制剂 凋亡 MDRl(p-gP) 胰腺癌 

分 类 号：R735.9[医药卫生—肿瘤]