自体富血小板血浆促进兔上颌缝牵张成骨的实验研究  

Study on the Effects of Platelet-rich Plasma on Maxillary Distraction Osteogenesis in Rabbits.

作　　者：叶亮[1] 曾新晨[2] 郭敏[3] 黎浩江[2] 王哲[4] 

机构地区：[1]中山大学光华口腔医院正畸科,广东广州510080 [2]中山大学中山医学院 [3]中山大学光华口腔医院颌面外科 [4]中山大学药学院实验中心

出　　处：《口腔医学研究》2008年第3期274-277,共4页Journal of Oral Science Research

摘　　要：目的:探讨自体富血小板血浆对兔上颌骨牵张成骨的影响。方法:将16只年轻健康家兔随机分为1周实验组(4只),1周对照组(4只),3周实验组(4只),3周对照组(4只)。分别在家兔左侧前颌缝的两侧置入钛钉(直径1.5mm),戴自制前牵引装置,1周实验组和1周对照组持续牵引1周,3周实验组和3周对照组持续牵引3周。试验组在牵张开始时将制得的富血小板血浆(platelet-rich plasma,PRP)与10%氯华钙、牛凝血酶以9∶1(v/v)比例混合,形成凝胶样物质注射至左侧前颌缝内。牵引结束后对骨缝两侧钛钉间距离的增加值以及组织学等指标进行观察以及相关统计学分析。结果:对照组试验组家兔上颌均向前移位。同对照组相比,实验组骨缝标志钉间距离增加值较大,新骨生成与矿化较快,血管分布较多,牵张间隙中骨小梁较为粗壮和成熟。结论:富血小板血浆有助于骨组织再生,对兔上颌骨牵张具有促进作用。Objective： To explore the effects of platelet-rich plasma （PRP） on maxillary distraction osteogenesis in rabbits. Methods ： Sixteen healthy young rabbits were randomly assigned into four groups ： the one week experimental group （n =4）, the one week control group（n=4）, the three weeks experimental group（n=4）and the three weeks control group （n=4）. Pairs of titanium bone markers were fixed on either side of suture of all animals. The self-restraint devices for distraction osteogenesis were placed on maxilla of 12 young rabbits. Distraction for 1 week was applied to the one week experimental group and the one week control group, and 3 weeks was applied to the three weeks experimental group and the three weeks control group. Both experimental groups were injected with gel-like substance formed by PRP mixed with 10% calcium chloride and thrombin of the ox at 9：1 （v/v） into the suture at the beginning of the lengthening time. The increase in the distance between two titanium bone markers and changes occurring in the tissue were observed in and around the suture after distraction. The data was analized statistically. Results： All animals appeared a progressviely increasing anteroir movement of maxllia. In contrast with the control group,the experimental group had a greater increase in the distance between two titanium bone markers and a higher speed of new bone formation and mineralization, and in the suture there was more vascular territory. In addition, the bone trabecula was thicker and more mature. Conclusion： Platelet-rich plasma could improve osteogenesis and promote maxillary distraction osteogenesis in rabbits.

关 键 词：富血小板血浆 牵引成骨技术 生长因子 

分 类 号：R782[医药卫生—口腔医学]