肝细胞肝癌中丙型肝炎病毒C区、E区、NS3区、NS4区抗原的表达  

Expression of C,E,NS3 and NS4 region antigens of Hepatitis C virus

作　　者：王理富[1,2,3] 李德富[1,2,3] 邓卓霖 郎淑慧[1,2,3] 丁志敏[1,2,3] 尹红章 冯建平[1,2,3] 

机构地区：[1]中国药品生物制品检定所 [2]卫生部生物技术产品检定及标准化重点实验室 [3]广西医科大学病理学教研室

出　　处：《中华微生物学和免疫学杂志》1997年第5期369-372,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家九五攻关课题

摘　　要：本文报道研究丙型肝炎病毒抗原在肝细胞肝癌组织内的定位分布情况。以丙型肝炎病毒（ＨＣＶ）的Ｃ、Ｅ、ＮＳ３、ＮＳ４区四种单克隆抗体用免疫组化方法检测了１３９例肝细胞肝癌（ＨＣＣ）的肝脏标本，结果总的阳性率为１５．１％。２１例阳性标本中，Ｃ区单抗检测阳性占８０．９％（１７／２１），Ｅ区占３３．３％（７／２１），ＮＳ３、ＮＳ４区均占５７．１％（１２／２１），表明应用多区段单抗有助于提高ＨＣＶ抗原的检出率。阳性物质主要存在于胞浆中，呈细、粗颗粒及块状，３例出现膜及膜下型，１例核内有阳性反应。ＨＣＶ感染与ＨＣＣ的发生发展有一定的关系。We studied the location of Hepatitis C virus antigens in hepatocellular carcinoma tissues.139 liver tissues specimens of hepatocellular carcinoma were assayed immunohistochemically with monoclonal antibodies to C,E,NS3 and NS4 antigens of Hepatitis C virus.The total Hepatitis C virus antigen positive ratio was 15 1%(21/139).Of 21 positive cases,positive immunostaining for C,E,NS3 and NS4 antigens was found in 80 9%(17/21),33 3%(7/21),57 1%(12/21) and 57 1%(12/21),respectively.This suggests that using several regions monoclonal antibodies contribute to find positive cases.Positive cells were mainly stained in the cytoplasms.Positive matters were morphologically,fine,rough granules.In 3 cases,positive matters were found in and under membrane,and 1 case in nucleus.In brief,Hepatitis C virus is thought to be associated with the occurrence and development of hepatocellular carcinoma.

关 键 词：丙型肝炎病毒 肝肿瘤 抗体 免疫组织化学 

分 类 号：R735.703[医药卫生—肿瘤] R392.11[医药卫生—临床医学]