非小细胞肺癌中Bmi-1表达情况的初步研究  

Preliminary investigation on expression of Bmi-1 in non-small-cellular lung cancer

作　　者：胡中华[1] 胡义德[1] 朱勇[1] 

机构地区：[1]第三军医大学附属新桥医院全军肿瘤研究所,重庆400037

出　　处：《中国肺癌杂志》2008年第1期107-109,共3页Chinese Journal of Lung Cancer

基　　金：国家自然科学基金项目(NO.307772144);重庆市自然科学研究基金项目(No.C51C-20055268)资助~~

摘　　要：背景与目的B细胞特异性莫洛氏鼠白血病病毒插人位点-1(B-cell-specific Moloney leukemia virus insertion site-1,Bmi-1)基因决定着细胞的定向分化与增殖,因此在细胞的恶性转化中发挥着重要的作用。本研究的目的是通过研究Bmi-1在非小细胞肺癌中的表达水平,初步分析其与非小细胞肺癌发生发展的可能相关性。方法采用免疫荧光和逆转录-多聚酶链式反应(RT-PCR)技术,对25例非小细胞肺癌及其相应癌旁正常肺组织中的Bmi-1进行检测。结果免疫荧光结果显示:Bmi-1在76%(19/25)的肺癌组织中癌细胞胞核部位呈阳性表达,癌旁组织均不表达Bmi-1。RT-PCR结果显示:Bmi-1在肺癌组织中的表达水平显著高于癌旁正常肺组织(P<0.05)。结论在非小细胞肺癌中,Bmi-1的表达升高可能与肺癌的发生发展有密切关系。Background and objective B-cell-specific Moloney leukemia virus insertion site 1 （Bmi-1） gene, which plays a critical role in directional differentiation and proliferation of cells, is significant in malignant transformation of cells. The aim of this study is to investigate the levels of Bmi-1 in non-small-cell lung cancer （NSCLC）, and to preliminarily analyze the correlation between Bmi-1 and the development of NSCLC tissue. Methotis The expression of Bmi-1 was detected in 25 NSCLC tissues as well as in the normal paracancerous lung tissues by immunofluorescence technique and reverse transcription-polymerase chain reaction （RT-PCR）. Results Immunofluorescently, the positive expression of Bmi-1 proteins was revealed in nucleus of tumor cells in 76%（ 19 / 25） of NSCLC tissue but not in paracancerous lung tissues. RT-PCR results showed that the expression of Bmi-1 in lung cancer tissues was significantly higher than that in paracancerous lung tissues （P〈0.05）. Conclusion Bmi-1 is over expressed in NSCLC and may be associated with the oncogenesis and development of lung cancer.

关 键 词：肺癌 人BMI1蛋白 病理学 

分 类 号：R734.2[医药卫生—肿瘤]