TCF-4在肺癌中高表达并与肺癌的进展有关  

High expression of TCF-4 positively correlated with lung cancer progression

作　　者：杨连赫[1] 徐洪涛[1] 王妍[1] 刘洋[1] 赵月[1] 戴顺东[1] 韦强[1] 苗原[1] 韩阳[1] 杨志强[1] 刘楠[1] 王恩华[1] 

机构地区：[1]中国医科大学基础医学院病理学教研室,中国医科大学第一临床学院病理科

出　　处：《中国肺癌杂志》2008年第2期214-219,共6页Chinese Journal of Lung Cancer

基　　金：国家自然科学基金(No.30670917)资助~~

摘　　要：背景与目的T细胞因子4(Tcellfactor4,TCF-4)是Wnt信号传导通路的重要分子,但其mRNA及蛋白在肺癌组织及细胞系中的表达情况和意义的相关研究较少。本研究的目的是检测TCF-4在肺癌组织和细胞系中的表达情况,并探讨其与肺癌的临床病理因素和生物学行为的关系。方法采用免疫组织化学(S-P法)检测120例肺鳞癌和肺腺癌患者及10例癌旁正常肺组织中TCF-4的表达;应用免疫荧光法检测肺癌细胞系及HBE(人正常支气管上皮)细胞系中TCF-4的表达水平及亚细胞定位;应用RT-PCR方法检测九种肺癌细胞系中TCF-4mRNA的表达水平。结果在120例肺鳞癌和肺腺癌组织标本中,96例为TCF-4高表达(80.0%),其中包括37例同时有细胞浆和核表达(30.8%)。TCF-4的高表达水平与患者的TNM分期正相关(P=0.022),10例癌旁正常肺组织无TCF-4表达。免疫荧光显示TCF-4蛋白在正常支气管上皮细胞系中表达极弱,而在肺癌细胞系中则出现明显的表达,主要定位于细胞核。TCF-4的mRNA在不同组织学类型的肺癌细胞系中的表达并不一致,巨细胞癌细胞系中相对较高,而腺癌细胞系中表达较低。结论TCF-4的高表达与肺癌的进展密切相关,有效抑制TCF-4在肺癌中的高表达可能成为肺癌治疗的新途径。Background and objective TCF-4 is an important downstream molecule of Wnt signaling pathway, but studies on the expression level and significance of TCF-4 in lung cancer were still limited. The aim of this study is to examine the expression level of TCF-4 in lung cancer tissues and cell lines, and analyze its relationship with clinicopathologic characteristics and biological behavior of lung cancers. Methods TCF-4 expression was examined in 120 lung cancer specimens and 10 corresponding normal lung specimens using immunohistochemistry （S-P method）. Immunofluorescence was used to examine the TCF-4 protein expression level and subcellular localization in lung cancer cells and HBE （human normal bronchi epithelium） cells. Expression levels of TCF：4 mRNA were examined using transcription-polymerase chain reaction （RT-PCR） in 9 lung cancer cell lines. Results Among 120 lung cancer specimens, 96 samples showed high expression of TCF-4 （80.0%）, including 37 samples showed TCF-4 expression both in cytoplasm and nuclei （30.8%）,TCF-4 were not detected in 10 corresponding normal lung specimens. TCF-4 expression level was positively correlated with TNM stages （P=0.022）. The fluorescence signal of TCF-4 protein was conspicuous in lung cancer cells, primarily in cell nuclei, but extremely low in HBE. TCF-4 mRNA expression levels were incompatible in different histology types, the expression level of TCF-4 was relatively high in giant cell carcinoma cells, but low in lung adencarcinoma cells. Conclusion High expression of TCF-4 positively correlated with lung cancer progression.

关 键 词：T细胞因子4 肺肿瘤 肿瘤标志物 免疫组织化学 免疫荧光 

分 类 号：R734.2[医药卫生—肿瘤]