Induction of nucleoside phosphorylase in Enterobacter aerogenes and enzymatic synthesis of adenine arabinoside  

作　　者：Xiao-kun WEI Qing-bao DING Lu ZHANG Yong-li GUO Lin OU Chang-lu WANG 

机构地区：[1]College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China [2]State Key Lab of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China [3]Shanghai Weiping Bioscience and Biotechnology Co., Ltd., Shanghai 200237, China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2008年第7期520-526,共7页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：Project (No. 07C26213101283) supported by the Innovation Fundfor Technology Based Firms from the Ministry of Science andTechnology of China

摘　　要：Nucleoside phosphorylases (NPases) were found to be induced in Enterobacter aerogenes DGO-04, and cytidine and cytidine 5′-monophosphate (CMP) were the best inducers. Five mmol/L to fifteen mmol/L cytidine or CMP could distinctly increase the activities of purine nucleoside phosphorylase (PNPase), uridine phosphorylase (UPase) and thymidine phosphorylase (TPase) when they were added into medium from 0 to 8 h. In the process of enzymatic synthesis of adenine arabinoside from adenine and uracil arabinoside with wet cells of Enterobacter aerogenes DGO-04 induced by cytidine or CMP, the reaction time could be shortened from 36 to 6 h. After enzymatic reaction the activity of NPase in the cells induced remained higher than that in the cells uninduced.Nucleoside phosphorylases （NPases） were found to be induced in Enterobacter aerogenes DGO-04, and cytidine and cytidine 5＇-monophosphate （CMP） were the best inducers. Five mmol/L to fifteen mmol/L cytidine or CMP could distinctly increase the activities ofpurine nucleoside phosphorylase （PNPase）, uridine phosphorylase （UPase） and thymidine phosphorylase （TPase） when they were added into medium from 0 to 8 h. In the process of enzymatic synthesis of adenine arabinoside from adenine and uracil arabinoside with wet cells ofEnterobacter aerogenes DGO-04 induced by cytidine or CMP, the reaction time could be shortened from 36 to 6 h. After enzymatic reaction the activity of NPase in the cells induced remained higher than that in the cells uninduced.

关 键 词：Nucleoside phosphorylase （NPase） Enterobacter aerogenes CYTIDINE Cytidine 5＇-monophosphate （CMP） Adenine arabinoside 

分 类 号：Q7[生物学—分子生物学]