共刺激分子CD28:CTLA4/B7在实验性自身免疫性重症肌无力中的作用  

Role of CD28:CTLA4/B7 costimulatory molecules in experimental autoimmune myasthenia gravis

作　　者：吴怀国[1] 彭晓江[1] 李军[1] 杨毅[2] 许文华[2] 任明山[2] 

机构地区：[1]安徽省立友谊医院神经内科,合肥230011 [2]安徽医科大学附属省立医院神经内科

出　　处：《临床神经病学杂志》2008年第3期210-212,共3页Journal of Clinical Neurology

基　　金：安徽省卫生厅基金资助(06B0033);安徽省自然科学基金资助(070413260X);安徽省人才开发基金重点资助(2005Z041)

摘　　要：目的研究共刺激分子CD28：CTLA4/B7在实验性自身免疫性重症肌无力（EAMG）发病中的作用。方法将雌性Lewis鼠随机分为EAMG组和对照组;EAMG组采用人工合成的Rα97-116肽段3次法免疫Lewis鼠,对照组同期注入等量的PBS;3次免疫接种后采用流式细胞术检测CD28、B7-2、B7-1、CTLA4在外周血、淋巴细胞、单核细胞中的表达。结果EAMG组大鼠成模率75%;与对照组比较,外周血CD28、B7-2B7-1、CTLA4的表达明显增加（P〈0.05-0.01）;EAMG组大鼠外周血CD28、CTLA4主要在淋巴细胞表达及B7-1、B7-2在淋巴细胞、单核细胞表达显著增加（P〈0.05-0.01）。结论EAMG大鼠存在共刺激分子CD28：CTLA4/B7表达异常,共刺激分子CD28：CTLA4/B7可能参与了EAMG的发生、发展。Objective To study pathogenesis role of C D28：CTLA4/B7 eostimulatory molecules in experimental autoimmune myasthenia gravis （EAMG）. Methods Female lewis rats were divided into EAMG group and control group. The rats were immunized thrice with Rα97-116 peptide in EAMG group, or only with phosphate buffer saline （PBS） in control group. 5 d after the third immunization, the expressions of CD28, CTLA4, B7-1, B7-2 on the surface of peripheral blood cells, lymphoeytes and monocytes were exaimed by flow cytometry. Results In EAMG group,the achievement ratio of EAMG model was 75% ; the expressions of CD28, CTLA4, B7-1, B7-2 on the surface of peripheral blood cells were significantly increased than those in control group （P 〈0.05 -0.01 ） ; the expressions of CD28, CTLA4 were mainly of lymphocytes, the expressions of B7-1, B7-2 were increased in lymphocytes and monocytes （ P 〈 0.05 - 0.01 ）. Condusions The expressions of CD28 ： CTLA4/B7 costimulatory molecules are abnormal in the rats with EAMG, which may participate in the occurrence and development of EAMG.

关 键 词：重症肌无力 共刺激分子 CD28:CTLA4/B7 

分 类 号：R746.1[医药卫生—神经病学与精神病学]