人神经母细胞瘤中bcl-2基因表达与肿瘤细胞凋亡间的关系及p16基因的表达  

Studies on gene expression of bcl2 and its correlation with tumor cell apoptosis as well as expression of p16 in human neuroblastoma

作　　者：折晓宁[1,2] 陈杰[1,2] 刘彤华[1,2] 张雷 李佩娟[1,2] 王德田[1,2] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院病理科 [2]北京儿童医院病理科

出　　处：《中华病理学杂志》1997年第5期289-292,共4页Chinese Journal of Pathology

基　　金：国家教委博士点基金;国家教委跨世纪人才基金;国家杰出青年基金

摘　　要：目的探讨人神经母细胞瘤中ｂｃｌ－２基因表达状况及与细胞凋亡的关系，以及ｐ１６基因的表达况状。方法应用原位杂交和免疫组化技术检测神经母细胞瘤中ｂｃｌ－２及ｐ１６两种基因的表达，并采用凋亡细胞原位检测方法对神经母细胞瘤组织切片中凋亡细胞数进行检测。结果原位杂交检测发现，２０例人神经母细胞瘤中，ｂｃｌ－２和ｐ１６基因表达阳性率均为９５％；免疫组化检测发现ｂｃｌ－２和ｐ１６蛋白表达阳性率均为１００％。χ２检验表明两种方法检测阳性率差异无显著性。凋亡细胞原位检测结果显示，肿瘤组织凋亡细胞数量随ｂｃｌ－２基因表达增强而减少。结论人神经母细胞瘤中ｂｃｌ－２基因有较普遍的表达，直接影响神经母细胞瘤中凋亡细胞的数量，ｐ１６基因未见明确的表达缺失。Objective To elucidate the relationship between bcl2 gene expression and the frequency of apoptosis of tumor cells, and study the expression of p16 tumor suppressor gene in neuroblastoma. Methods In situ hybridization and immunohistochemistry methods were used to study the frequencies of expression of bcl2 and p16 genes in 20 cases of neuroblastoma. Meanwhile, an in situ apoptotic cell detection method was adopted to detect the apoptotic cells in these tumors, and the number of apoptotic cells was compared with the bcl2 gene expression in each case. Results In situ hybridization revealed that the positive frequencies of both bcl2 and p16 gene expression in 20 neuroblastoma specimens were 95%, and the expression rates at the protein level of these 2 gene products as detected by immunohistochemistry were both 100%. There was no significant difference between the positive rates obtained by these 2 methods. Comparing the bcl2 expression and apoptotic cell number in these specimens, we found that the apoptotic cell number increased as the level of bcl2 expression decreased. Conclusions The bcl2 gene was expressed in most of human neuroblastomas. The reverse correlation of bcl2 expression and tumor cell apoptosis further confirms that bcl2 as an important gene inhibiting cell apoptosis may indirectly promote the carcinogenesis of neuroblastoma. It seems that there was no significant loss of p16 gene expression in these cases.

关 键 词：神经母细胞瘤 基因表达 脱噬作用 

分 类 号：R730.264[医药卫生—肿瘤]