保肝解毒颗粒对雷公藤多苷致急性肝损伤小鼠自由基脂质过氧化反应的影响  

Effects of Baogan Jiedu Granule on lipid peroxidation of free radicals in acute liver injury mice induced by tripterygium glycosides

作　　者：禄保平[1] 胡丹华[1] 杨晓慧[1] 

机构地区：[1]河南中医学院肝病研究所,河南郑州450008

出　　处：《西安交通大学学报（医学版）》2008年第3期333-335,共3页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：河南省科技攻关计划项目(No.0324410026);河南省高等学校青年骨干教师资助计划项目(No.教高[2007]335号)

摘　　要：目的探讨保肝解毒颗粒对雷公藤多苷致急性肝损伤小鼠自由基脂质过氧化反应的影响。方法60只小鼠随机分为模型组、保肝解毒颗粒大、中、小剂量组、甘利欣对照组和空白对照组,分别以相应剂量药物灌胃5 d,再以雷公藤多苷灌胃造模,检测小鼠血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)水平。结果模型组血清SOD、GSH-Px水平明显降低,MDA水平明显升高;保肝解毒颗粒各剂量组均可有效降低血清ALT、AST和MDA水平,显著提高GSH-Px水平,大剂量组尚可显著提高血清SOD水平,与模型组比较差异均有统计学意义(P<0.05)。结论保肝解毒颗粒具有明显的降酶保肝作用和抗自由基脂质过氧化作用。Objective To explore the effects of Baogan Jiedu Granule on lipid peroxidation of free radicals in acute liver injury mice induced by tripterygium glycosides. Methods Totally 60 mice were randomly divided into tripterygium glycosides model group, large-dose,： medium-dose and small-dose groups of Baogan Jiedu Granule, Ganlixin group and control group. Each group was respectively perfused with the corresponding drug for 5 days and tripterygium glycosides were perfused once to make the model. The serum levels of ALT, AST, SOD, MDA and GSH-Px were measured. Results The serum levels of SOD and GSH-Px in model group decreased and MDA rose obviously. Compared with model group, Baogan Jiedu Granule of all doses could effectively decrease ALT, AST and MDA level and obviously improve GSH-Px level in each group. In addition, in the large-dose group SOD level was markedly improved （P〈0.05）. Conclusion Baogan Jiedu Granule has obvious therapeutic effects on liver injury through decreasing serum transaminase level and anti-lipid peroxidation of free radicals.

关 键 词：肝脏疾病 中医药疗法 动物模型 雷公藤多苷 脂质过氧化 保肝解毒颗粒 

分 类 号：R575.29[医药卫生—消化系统]