大豆异黄酮对氧化损伤血管内皮细凋亡作用  

Inhibitive effects of soybean isoflavone on apoptosis of vascular endothelial cells following oxidative injury in vitro

作　　者：黄国伟[1] 曹小红[1] 刘英华[2] 常红[3] 刘莉[3] 任大林[3] 

机构地区：[1]天津科技大学食品工程与生物技术学院天津市食品营养与安全重点实验室,天津300457 [2]中国人民解放军总医院营养科 [3]天津医科大学公共卫生学院

出　　处：《中国公共卫生》2008年第7期830-831,共2页Chinese Journal of Public Health

摘　　要：目的探讨大豆异黄酮(SI)对氧化损伤血管内皮细胞凋亡作用。方法体外培养血管内皮细胞,采用氧化低密度脂蛋白(ox-LDL)进行氧化损伤,将细胞分为6组,即空白对照组、氧化损伤对照组(ox-LDL)、氧化损伤加入维生素E对照组(VE+ox-LDL)、氧化损伤加入SI低、中、高剂量组(SI-L+ox-LDL、SI-M+ox-LDL、SI-H+ox-LDL)。将ox-LDL作用于预先加入VE或不同剂量SI孵育24 h的内皮细胞,继续培养24 h,测定细胞四甲基偶氮噻唑蓝(MTT)吸光度(A)值、乳酸脱氢酶(LDH)释放情况、细胞凋亡指数(AI)及形态学观察。结果空白对照组、VE+ox-LDL及(SI-L、M、H)+ox-LDL组细胞MTT(A)明显高于ox-LDL组(P<0.01);空白对照组、VE+ox-LDL及(SI-L、M、H)+ox-LDL组细胞LDH释放百分比及AI均明显低于ox-LDL组(P<0.01)。结论SI对体外氧化损伤血管内皮细胞凋亡具有明显的抑制作用。Objective To study the effects of soybean isoflavone （SI） on the apoptosis of vascular endothelial cells following oxidative injury in vitro. Methods The vascular endothelial cells were cultured in vitro and divided into 6 groups, vascular control, oxidized low density lipoprotein （ox - LDL）, VE ＋ ox - LDL, SI - Low（SI - L） ox - LDL, SI - Middle（SI - M） ox- LDL, SI - High（SI - H） ox- LDL groups. The vein endothelial cells were incubated with ox- LDL for 24 hours after incubated with VE on different doses of SI for 24 hours. The levels of MTT （A）, LDH releasing and AI of cells were measured and their morphologic characters were observed. Results The MTT （A） levels of control, VE ＋ ox- LDL and （SI - L, M, H） ＋ ox - LDL groups were significantly higher than those of ox - LDL group （ P 〈 0.01 ）. The levels of releasing percentage of LDH and apoptosis index in control, VE ＋ ox- LDL and （SI- L, M, H） ＋ ox- LDL groups were significantly lower than those in ox - LDL group（ P 〈 0.01 ）. Conclusion SI can inhibit apoptosis of vascular endothelia cells following oxidative injury in vitro.

关 键 词：大豆异黄酮 血管内皮细胞 凋亡 

分 类 号：R151.3[医药卫生—营养与食品卫生学]