二烯丙基二硫诱导HL-60细胞系凋亡及其与Caspase-3的关系  

Apoptosis and Activation of Caspase-3 in Leukemia HL-60 Cells Induced by Diallyl Disulfide

作　　者：易岚[1,2] 苏琦[1] 李国庆[2] 秦志峰[2] 贺庆芝[2] 刘运莲[2] 殷杰[2] 杨露青[2] 

机构地区：[1]南华大学肿瘤研究所,湖南衡阳421001 [2]南华大学生命科学与技术学院

出　　处：《南华大学学报（医学版）》2008年第1期32-35,共4页Journal of Nanhua University(Medical Edition)

基　　金：湖南省教育厅资助项目(06C696)

摘　　要：目的研究二烯丙基二硫(DADS)诱导白血病细胞凋亡及其机制。方法实验分对照组、DADS处理组、Ac-DEVD-CHO预处理组,进行体外细胞培养,采用形态学观察、MTT法、流式细胞术、DNA凝胶电泳等方法,进行细胞形态学观察、细胞增殖抑制作用研究,检测细胞凋亡率、Caspase-3活性以及DNA Ladder检测。结果DADS诱导前后,HL-60细胞形态学发生明显改变,DADS以剂量依赖的方式抑制细胞的生长,在DADS诱导HL-60细胞凋亡过程中有Caspase-3的活化,且与剂量和作用时间呈依赖关系,Caspase-3抑制剂Ac-DEVD-CHO能抑制DADS诱导HL-60细胞凋亡。结论DADS能有效地诱导HL-60细胞凋亡,其过程与Caspase-3活化相关。Objective To investigate the apoptosis in human leukemia HL- 60 cells induced by DADS, and study the mechanism. Methods HL - 60 cells were treated with different concentrations of DADS. After incubation of HL - 60 cells with DADS, the effects of DADS on HL- 60 cell growth inhibition were estimated by morphology and MTT assay, flow cytometry was used to determine the induction of apoptosis and the expression of actived Caspase - 3, DNA agarose electrophoresis was used to determine the induction of apoptosis. Results After HL - 60 cells were exposed to DADS, the typical apoptotic morphological changes were observed under the light microscope and inverted microscope, DADS inhibited the growth of HL- 60 cells in a dose - dependent manner, when HL - 60 cells induced by DADS the expression of actived Caspase - 3 was increased in a dose - and time - depen- dent manner. Pretreatment with Caspase - 3 specific inhibitor Ac - DEVD - CHO prevented DADS induced apoptosis. Conclusion DADS can efficiently induce apoptosis in HL60 cells and the activation of Caspase - 3 may contribute to the effect.

关 键 词：人白血病细胞 二烯丙基二硫 凋亡 CASPASE-3 

分 类 号：R733.7[医药卫生—肿瘤]