不同盐析剂对羔羊皱胃酶盐析效果的影响  

Influences of different salting-out reagents on effect of kid rennet

作　　者：吴海玥[1] 张富新[1] 昝林森[2] 李传扬[1] 

机构地区：[1]陕西师范大学食品工程与营养科学学院,陕西西安710062 [2]西北农林科技大学动物科学学院,陕西杨凌712100

出　　处：《陕西师范大学学报（自然科学版）》2008年第4期106-108,共3页Journal of Shaanxi Normal University：Natural Science Edition

基　　金：国家“十一五”科技支撑计划奶业重大专项研究基金资助项目(2006BAD04A11)

摘　　要：以NaCl、Na2SO4、MgSO4和（NH4）2SO4为盐析剂，对影响羔羊皱胃酶的盐析饱和度、盐析pH、盐析温度和盐析时间进行了研究．结果表明，不同盐析剂对羔羊皱胃酶盐析效果各不相同，（NH4）2SO4是盐析羔羊皱胃酶较适宜的盐析剂．（NH4）2SO4饱和度95％、盐析pH值为4．60、盐析温度为4℃下盐析14-20h，盐析活性回收率可达到92．86％．Using NaCl, Na2SO4 、 MgSO4 and （NH4） 2 SO4 as salting-out reagents, some factors, such as saturation pH, temperature and time, influencing salting-out effects on kid rennet were studied systematically. The results showed that different salting-out reagents have different effect on salting-out and （NH4）2SO4 is a optimal salting-out reagent,by which enzyme activity recovery can reached 92.86% under （NH4）2SO4 saturation 95%, pH 4.60, temperature 4℃ and time 14-20 hours.

关 键 词：盐析剂 羔羊皱胃酶 盐析条件 

分 类 号：O426.1[理学—声学]