乳腺小黏蛋白在检测乳腺癌患者外周血微转移中的意义  

Detection of SBEM-mRNA level in peripheral blood of patients with breast cancer and its clinical significance

作　　者：仲雷[1] 张建国[1] 郭宝良[1] 杨学伟[1] 单明[1] 

机构地区：[1]哈尔滨医科大学附属二院乳腺外科,哈尔滨150086

出　　处：《中华乳腺病杂志（电子版）》2008年第1期24-27,共4页Chinese Journal of Breast Disease(Electronic Edition)

基　　金：国家自然科学基金资助项目(30672420);黑龙江省科技厅攻关项目(GB05C402-01)

摘　　要：目的探讨乳腺小黏蛋白(small breastepithelial mucin,SBEM)mRNA作为检测乳腺癌患者外周血微转移指标的临床意义。方法用巢式逆转录-聚合酶链反应(Nested-RT-PCR)法检测22例女性胃癌、16例结/直肠癌、107例乳腺癌、40例乳腺纤维腺瘤患者和27例健康志愿者外周血中SBEM-mRNA的表达情况。结果107例乳腺癌患者外周血中SBEM-mRNA有57例表达,阳性率为53.27%,其余对照组中SBEM-mRNA表达均为阴性。两组比较差异有统计学意义(P<0.05)。SBEM-mRNA的阳性表达与患者的淋巴结转移状况和TNM分期均有相关性(P均<0.01);而与患者的年龄、原发肿瘤大小、病理类型、ER、PR等无相关性(P>0.05)。结论SBEM-mRNA特异表达于乳腺癌患者的外周血,可能成为检测乳腺癌患者外周血中微转移和判断预后的指标之一。Objective To evaluate the clinical significance of SBEM （small breast epithelial mucin） mRNA as a mierometastases marker in peripheral blood of breast cancer. Methods The SBEM- mRNA level in peripheral blood was detected in 22 patients with gastric cancer, 16 colon/rectal carcinoma, 107 breast cancer, 40 breast fibroadenoma and 27 healthy volunteers as controls using rested reverse transcription-polymerase chain reaction （Nested-RT-PCR）. Results The positive expression rate of SBEM- mRNA in peripheral blood of breast cancer patients was 53.27% （57/107 cases）, and no positive SBEM- mRNA was found in other controls. There was a significant difference between the two groups （ P 〈 0.05 ）. SBEM-mRNA expression was correlated with axillary lymph nodes status and TNM （ P 〈 0. 01 ）, but uncorrelated with other clinical parameters （age, tumor size, histopathlogy types, estrogen receptor and progesterone receptor）. Conclusion SBEM-mRNA is sensitive and specific for breast cancer, and the positive expression in peripheral blood may be a marker in detecting micrometastases and judging prognosis.

关 键 词：乳腺癌 微转移 巢式逆转录聚合酶链反应 SBEM—mRNA 

分 类 号：R737.9[医药卫生—肿瘤]