痰瘀共治对大鼠动脉粥样硬化斑块的影响及其可能机制  

Influence of Removing Both Phlegm and Blood Stasis on Atherosclerotic Plaques in Rats and Potential Mechanism

作　　者：刘艳[1] 王坤根[1] 叶武[1] 陈隽妍[2] 陈杰[2] 华军益[1] 蔡宏文[1] 毛威[1] 倪桂宝[1] 李剑钢[1] 

机构地区：[1]浙江中医药大学附属第一临床医学院,浙江杭州310006 [2]浙江中医药大学,浙江杭州310006

出　　处：《中华中医药学刊》2008年第7期1466-1469,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省中医药科技计划重点研究项目(2006Z004)

摘　　要：目的:探讨痰瘀共治方对大鼠动脉粥样硬化(atherosclerosis,AS)斑块的影响及其可能机制。方法:12周龄雄性Wistar大鼠85只,随机分为5组:①正常组(the control group,C,n=20),②模型组(the model group,M,n=20),③罗格列酮组(the Rosiglitazone group,RSG,n=15),④痰瘀共治低剂量组(TYD,n=15),⑤痰瘀共治高剂量组(TYG,n=15)。采用高脂饲料喂饲法加维生素D3负荷法复制大鼠AS模型,6周后取C组及M组大鼠各5只,以主动脉HE染色切片发现AS斑块作为造模成功指标。造模成功后除正常组喂饲基础饲料,余组继续喂饲高脂饲料,并分别加予生理盐水[10mL/(kg·d)]、罗格列酮(3mg/kg)、痰瘀共治方流浸膏高剂量(0.8g/mL)、痰瘀共治方流浸膏低剂量(1.6g/mL)灌胃治疗6周后处死,HE染色观察大鼠主动脉形态学变化,免疫组织化学染色法观察主动脉过氧化物酶体增值物激活受体γ(PPARγ)蛋白表达的变化,并检测空腹血糖(fasting plasma glu-cose,FPG)、空腹胰岛素(fasting insulin,FINS),计算胰岛素抵抗指数(HOMA-IR)。结果:高脂饮食加维生素D3负荷6周后造模成功,光镜下观察可见主动脉典型的粥样硬化斑块;灌胃治疗6周后,与M组比,各治疗组大鼠FPG、FISN、HOMA-IR水平均降低(P<0.05或P<0.01),HE染色见斑块明显消退,PPARγ蛋白表达平均光密度值明显减弱,以TYG组(1.08±0.03)作用显著(P=0.0025),低于TYD组(2.84±0.07)(P=0.034),与RSG组(1.12±0.05)比差异亦没有统计学意义(P=0.427),同时各治疗组与C组比差异没有统计学意义(P>0.05)。结论:痰瘀共治方可能通过激活PPARγ,改善胰岛素抵抗起到抗动脉粥样硬化的作用。Objective：To observe the influence of Removing Both Phlegm and Blood stasis on atherosclerotic plaques in rats and the potential mechanism. Methods ： To randomly divide 85 Wistar rats into 5 groups ： the normal group （ n = 20, fed with basic diet for 12 weeks） , the model group （n = 20） , rosiglitazone group （n = 15 ） , low -dose recipe of removing both phlegm and blood stasis group （ n = 15 ） and high - dose recipe of removing both phlegm and blood stasis group （ n = 15 ）. After 6 weeks,5 rats from the normal group and 5 rats from the model group were sacrificed. Atherosclerosis plaque was examined in aorta by optical microscope with Hematoxylln and eosin （HE） staining, which was the successful index of model establishment. The rest four groups were intraperitoneal injected with single dose of vitamin D3 （400000IU/kg） and were fed with high fat diet for 12 weeks. From the 7th week on,groups were intragastric administrated with normal saline [ 10mL/（ kg·d）] ,rosiglitazone （3mg/kg） ,low- dose recipe of removing both phlegm and blood stasis liquid extract （0.8g/mL） ,high -dose recipe of removing both phlegm and blood stasis liquid extract （1.6g/mL） for the next 6 weeks. The aorta histological changes was examined with HE staining and peroxisome proliferator activated receptor - γ （PARγ） protein expression was observed by immunohistochemical method. The level of fasting plasma glucose （ FPG）, fasting insulin （FINS） were detected, and insulin resistance index （HOMA -IR） was calculated accordingly. Results：Injecting single dose of vitamin D3 40（0）OIU/kg with loading of high fat diet for 6 weeks,atherosclerosis model in rats was established with the typital plaque. After 6 weeks intragastric administration, compared with the model group rats, rosiglitazone,low - dose recipe of removing both pldegm and blood stasis liquid extract and high - dose recipe of removing both phlegm and blood stasis liquid extract could significantly reduce the level of

关 键 词：痰瘀共治 动脉粥样硬化 大鼠 过氧化物酶体增殖物激活受体Γ 

分 类 号：R-33[医药卫生]