PPARγC161→T基因多态性对代谢综合征和膳食因素的影响  

Association of peroxisome proliferator-activated -receptors- gamma CI61→T gene polymorphism with metabolic syndrome and dietary predisposition

作　　者：刘明[1] 郭红卫[1] 万文涛[1] 骆璇[1] 张世秀[1] 薛琨[1] 马易怡[1] 

机构地区：[1]复旦大学公共卫生学院营养与食品卫生教研室与公共卫生安全教育部重点实验室,上海200032

出　　处：《中华预防医学杂志》2008年第7期494-498,共5页Chinese Journal of Preventive Medicine

基　　金：达能营养中心膳食营养研究与宣教基金（200706）

摘　　要：目的探讨过氧化物酶体增殖物激活受体γ（peroxisome proliferator-activatedl-eceptors-gamma，PPARγ）基因C161→T多态性在人群中的分布及其与代谢综合征和膳食因素的关系。方法从血凝块中提取DNA，用聚合酶链反应和限制性片段长度多态性方法（PCR—RFLP）检测上海224例成年代谢综合征人群以及224名对照人群的PPARγ基因C161→T位点多态性；对研究对象进行体格检查、膳食调查及血清生化指标测定。结果CC、CT和TT三种基因型的频率分别为32．4％、49．6％和18．O％，符合Hardy—Weinberg定律。代谢综合征人群和正常组人群的基因型CC、CT和TT及等位基因C和T构成比差异无统计学意义，三种基因型及等位基因C和T在性别上差异无统计学意义。三种基因型人群中体质指数、腰围和臀围差异有统计学意义，其中CT基因型的水平最高。代谢综合征人群不同基因型人群中，蛋白质与血清甘油三酯水平的负相关性有统计学意义。结论PPARγ基因C161→T位点多态性与体质指数、腰围和臀围有关；该位点多态性可能会影响膳食对血清甘油三酯的易感性。Objective To study the genotype frequencies of peroxisome proliferators-activated -receptors-gamma C161→T gene and its possible association with the metabolic syndrome and dietary intakes. Methods The PCR-PFLP method was used to detect the polymorphism of PPARγC161→T gene of 224 adults with metabolic syndrome and 224 normal adults in Shanghai. Their physical examinations, dietary investigation and the serum biochemistry were analyzed. Results （ 1 ） The genotype frequencies of PPARγ C161→T CC, CT and TTwere 32.4%, 49. 6% and 18.0% respectively, which were in agreement with Hardy-Weinberg equilibrium. There was no significant difference in the distribution of genotypes or allele between the metabolic syndrome group and the control group, and the result was the same between male and female subjects. （2） The levels of body mass index, waist width and hip width were significantly different among three genotypes groups. Subjects of the CT genotype had the highest levels. （3） There was significant difference in the negative correlation with the intake of protein and serum TG levels in the metabolic syndrome group. Conclusion The results suggested PPARγ gene C161→T should be associated with body mass index,waist width and hip width. It might contribute to the heterogeneity in diet response to TG.

关 键 词：过氧化物酶体增殖物激活受体-Γ 基因多态性 代谢综合征 

分 类 号：R686[医药卫生—骨科学]