谷胱甘肽过氧化物酶1和硫氧还蛋白还原酶2遗传变异与胃癌易感性的相关性  

Association of genetic polymorphisms in selenoprotein GPX1 and TXNRD2 with genetic susceptibility of gastric cancer

作　　者：王佳[1] 孙瞳 杨明 林东昕 谭文 李可基[1] 肖颖[1] 

机构地区：[1]北京大学医学部营养与食品卫生系,100083 [2]中国医学科学院肿瘤研究所病因与癌变研究室

出　　处：《中华预防医学杂志》2008年第7期511-514,共4页Chinese Journal of Preventive Medicine

基　　金：国家“973”计划（2004CB518701）

摘　　要：目的探讨谷胱甘肽过氧化物酶1（GPX1）第198位氨基酸Pro→Leu和硫氧还蛋白还原酶2（TXNRD2）第370位氨基酸Lys→Arg遗传变异单独或联合与胃癌易感性的关系。方法采用聚合酶链式反应．限制性片段长度多态性方法，检测361例胃癌患者和363例无肿瘤正常对照的基因型，用logistic多因素分析计算各基因型以及基因-基因交互作用与胃癌易感性的相关性。结果GPX1198Pro→Leu和TXNRD2370Lys→Arg变异单独与胃癌易感性无关。但GPX1和TXNR2基因型存在基因-基因交互作用，携带GPX1198Pro／Pro和TXNRD2370Arg／Arg这种保护基因型者患胃癌的风险比携带其他危险基因型者低62％（OR=0．38，95％C／：0．26—0．55，P〈0．001）。结论GPX1198Pro→Leu和TXNRD2370Lys→Arg基因型可能与胃癌的易感性相关。Objective This study examined whether the two polymorphisms of GPX1 （ 198Pro→ Leu） and TXNRD2 （370Lys→Arg） contributed alone or in combination, to the risk of gastric cancer development. Methods A total of 361 patients with gastric cancer and 363 cancer-free controls were recruited and their genotypes of the two polymorphisms were determined by polymerase chain reaction-based restrictive fragment length polymorphism （PCR-RFLP） method. Odds ratio （ OR ） and 95% confidence interval （CI） were computed using unconditional logistic regression model. Results GPX1 and TXNRD2 polymorphisms individually were not associated with the risk of gastric cancer. Gene-gene interaction of GPX1 and TXNRD2 polymorphisms decreased the risk of gastric cancer. Carrying the protective genotype might decrease the risk at 62% （ OR = 0. 38,95% CI = 0. 26-0. 55, P 〈 0. 001 ） as compared with the risk genotype. Conclusion The GPX1 198 Pro/Pro and TXNRD2 370Arg/Arg genotypes might be associated with the genetic susceptibility of gastric cancer.

关 键 词：胃肿瘤 谷胱甘肽过氧化酶 硫氧还蛋白还原酶 多态性 单核苷酸 

分 类 号：R686[医药卫生—骨科学]