甘蓝型油菜显性核雄性不育基因的AFLP标记鉴定  

Identification by AFLP marker for dominant genic male sterility gene in Brassica napus L.

作　　者：程勇[1] 邹崇顺[1] 李云昌[1] 李桂英[1] 郑普英[1] 张学昆[1] 刘胜毅[1] 

机构地区：[1]中国农业科学院油料作物研究所,湖北武汉430062

出　　处：《中国油料作物学报》2008年第2期148-151,156,共5页Chinese Journal of Oil Crop Sciences

基　　金：湖北省自然科学基金项目(2006ABA359);国家863计划(2006AA10Z1C2)

摘　　要：甘蓝型油菜双基因显性细胞核雄性不育系统受一对显性不育基因和一对显性上位抑制基因共同控制,不育单株的基因型为MsMsrfrf和Msmsrfrf。为了在育种过程中高效筛选后代单株基因型,本研究利用一个显性细胞核雄性不育杂合两型系70056AB(Msmsrfrf×msmsrfrf)构建的兄妹交分离群体,采用AFLP技术,在480对AFLP引物中筛选到一个与不育基因紧密连锁的分子标记P9M14-130。利用P9M14-130引物对70056AB的88个单株进行检测,发现该标记与雄性不育基因间的交换率为1.1%。In Brassica napus, DGMS was controlled by a dominant sterility gene and a dominant epistatic gene, and the genotype of sterile plants were MsMsrfrf and Msmsrfrf. To effectively screen progeny individuals, an AFLP molecular marker P9M14 - 130 was found in the 70056AB sib - mating population among 480 pair primers. The marker was proved to be strongly linked to the sterile gene with the recombination value of 1.1% among 88 investigated plants.

关 键 词：甘蓝型油菜 显性核不育基因 AFLP标记 

分 类 号：S565.403[农业科学—作物学]