含锰磁性纳米粒子固定脂肪酶的研究  

Study on Immobilization of Lipase with Magnetic Nanoparticles Containing Mn(Ⅱ)

作　　者：王燕佳 蒋惠亮 殷伟庆 

机构地区：[1]江南大学化学与材料工程学院,江苏无锡214122 [2]连云港市环境保护局,江苏连云港222001

出　　处：《淮海工学院学报（自然科学版）》2008年第2期51-54,共4页Journal of Huaihai Institute of Technology:Natural Sciences Edition

基　　金：浙江省科技攻关项目(021101047)

摘　　要：用无皂乳液聚合制备了P(AM-co-St)-Mn(Ⅱ)磁性高分子纳米微球,在此基础上通过共价键合固定脂肪酶。结果表明,固定脂肪酶后的磁性纳米微球具有优异的磁分离能力;锰离子对固定化酶有明显的激活作用,当锰离子质量分数为1.4%时,偶联率和活力回收率分别提高88%和71%;脂肪酶被固定化后其pH稳定性比自由酶明显提高。Based on the preparation of P（AM-co-St）-Mn（ Ⅱ ） magnetic nanoparticles by emulsifier free emulsion, lipase was immobilized on the prepared nanoparticles with covalent linkage. It was found that immobilized lipase magnetic nanoparticles possessed excellent ability of magnetic sepa-ration. Mn（ Ⅱ ） could activate the immobilized lipase, and when the weight ratio of Mn（ Ⅱ ） was 1.4%, the coupled yield and activity of the immobilized lipase was increased by 88% and 71% respectively. Compared with free lipase, the pH and operation stabilities of immobilized lipase were increased remarkably.

关 键 词：磁性纳米粒子 Mn(Ⅱ) 固定 脂肪酶 

分 类 号：O646[理学—物理化学]