苯扎贝特对ox-LDL诱导内皮细胞凋亡基因Bcl-2/Bax的影响  

Effects of bezafibrate on apoptosis gene Bcl-2/Bax in cultured endothelial cells induced by ox-LDL

作　　者：申晓彧[1] 薛丽霞[1] 屈巧芳[1] 曾秋棠[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院心内科,武汉430022

出　　处：《山西医科大学学报》2008年第7期596-599,共4页Journal of Shanxi Medical University

基　　金：太原市科技局科技兴市基金资助项目(0703046)

摘　　要：目的观察苯扎贝特对氧化型低密度脂蛋白(ox-LDL)诱导人脐静脉内皮细胞凋亡基因Bcl-2/Bax的影响。方法体外培养人脐静脉内皮细胞(HUVECs),根据实验要求分为正常对照组、ox-LDL组、低浓度苯扎贝特(50μmol/L)组、中浓度苯扎贝特(100μmol/L)组、高浓度苯扎贝特(200μmol/L)组。RT-PCR观察各组凋亡基因Bcl-2、Bax及Bcl-2/BaxmRNA的变化。结果与正常对照组比较,ox-LDL组凋亡基因Bcl-2表达下降(P<0.05),凋亡基因Bax表达增加(P<0.05),Bcl-2/Bax比值下降(P<0.05);不同浓度苯扎贝特组与ox-LDL组比较,Bcl-2表达增加(P<0.05),Bax表达降低(P<0.05),Bcl-2/Bax上调(P<0.05),且呈浓度效应依赖关系。结论ox-LDL可引起内皮细胞抗凋亡基因Bcl-2表达下调,凋亡基因Bax表达上调,Bcl-2和Bax比值下降,从而引起内皮细胞凋亡增加;苯扎贝特可通过上调Bcl-2与Bax的比值抑制ox-LDL引起的内皮细胞凋亡,起到抗动脉粥样硬化作用。Objective To observe the effect of bezafibrate on the apoptosis gene Bcl-2/Bax in cultured human umbilical vein endothelial cells（HUVECs）activated by oxidized low density lipoprotein（ox-LDL）. Methods HUVECs were cultured in vitro ,and divided into five groups： control group, ox-LDL group, 50 μmol/L bezafibrate ＋ ox-LDL group, 100 μmol/L bezafibrate ＋ ox-LDL group,200 pmol/L bezafibrate ＋ ox-LDL group. The expression of Bcl-2 mRNA and Bax mRNA was assayed by RT-PCR. Results Compared with control group, the expression of Bcl-2 decreased（ P 〈 0.05）, the expression of Bax increased（P 〈 0.05）, Bcl-2/Bax ratio decreased in ox-LDL group（ P 〈 0.05）. Bezafibrate could increase the expression of Bcl-2, decrease the expression of Bax, increase the Bcl-2/Bax ratio in a concentration-dependent manner（P〈0.05）. Conclusion ox-LDL could induce the endothelial cell apoptosis by decreasing the expression of Bcl-2, increasing the expression of Bax, decreasing the Bcl-2/Bax ratio. Bezafibrate can decrease the ox-LDL-induced apoptosis of HUVECs by increasing Bcl-2/Bax ratio, which may be responsible for bezafibrate to prevent and treat atherosclerosis.

关 键 词：苯扎贝特 内皮细胞 氧化型低密度脂蛋白 Bcl-2 Bax 

分 类 号：R363.21[医药卫生—病理学]