丁酸钠对胰腺癌BxPC-3细胞凋亡机制的影响  

Effects of Sodium Butyrate on the Apoptosis of Human Pancreatic Cancer Cell Line BxPC-3

作　　者：王志英[1] 潘定宇[2] 汪群[2] 

机构地区：[1]武汉大学公共卫生学院 [2]武汉大学中南医院普外科,湖北武汉430071

出　　处：《武汉大学学报（医学版）》2008年第4期454-457,471,共5页Medical Journal of Wuhan University

摘　　要：目的:探讨丁酸钠对胰腺癌BxPC-3细胞凋亡的影响及其作用机制。方法:采用MTT法观察不同浓度丁酸钠对BxPC-3细胞增殖的抑制作用;应用流式细胞仪检测丁酸钠作用于BxPC-3细胞后的凋亡情况;TRAP-ELISA法检测细胞端粒酶活性的变化;RT-PCR技术分析人端粒酶逆转录酶(hTERT)、bcl-2 mRNA的表达水平。结果:丁酸钠作用BxPC-3细胞能明显抑制细胞增殖,其作用具有时间和剂量依赖性。丁酸钠处理72 h的BxPC-3细胞凋亡率明显提高(P<0.01),S期细胞比例显著下降(P<0.05),G0/G1期细胞比例显著升高(P<0.05)。经丁酸钠处理的实验组端粒酶活性为0.96±0.12,未经丁酸钠处理的对照组端粒酶活性为4.18±0.34,二者之间差异有显著性(P<0.05)。丁酸钠处理的实验组hTERT mRNA水平为0.168±0.045,与对照组0.685±0.141比较差异有统计学意义(P<0.01),bcl-2实验组水平为0.138±0.034,与对照组0.715±0.026比较有统计学差异。结论:丁酸钠具有强烈诱导胰腺癌BxPC-3细胞凋亡的作用,其发生机制与丁酸钠下调hTERT Bcl-2 mRNA表达水平直接相关。Objective： To explore the effect of sodium butyrate on the cell apoptosis of human pancreatic cancer cell line BxPC-3 and its mechanism. Methods： The proliferating activity of BxPC-3 cells was observed by MTT assay. Flowcytometry was used to assess the apoptosis after sodium butyrate treatment. And TRAP-ELISA was applied to analyze the telomerase activity of human pancreatic cancer cell. In addition, the expressions of telomerase hTERT mRNA and Bcl-2 mRNA were confirmed by RT-PCR technology. Results： A time and dose dependent inhibition was remarkably confirmed in BxPC-3 cells. As compared with that in control group, apoptosis rate of BxPC-3 cells in sodium butyrate group was increased obviously at 72 h （P〈0.01）. The percentage of S phase decreased significantly, while the percentage of G0/G1 phase increased markedly （both P〈0.05）. The activity of telomerase in research group was significantly lower than that in control group （0. 96±0. 12 vs 4. 18±0. 34, P〈0.05）. Furthermore, the expression level of hTERT mRNA and bcl-2 mRNA in sodium butyrate group was significantly lower （0. 168 ± 0.045 vs 0. 685±0.141, 0. 138± 0.034 vs 0.715±0.026, respectively, both P〈0.01）. Conclusion, Sodium butyrate can greatly induce apoptosis ofpancreatic cancer BxPC-3 cell line, and the decline of expression level of hTERT Bcl-2 mRNA might contribute to the procedure of apoptosis.

关 键 词：丁酸钠 胰腺癌 BXPC-3细胞 凋亡 

分 类 号：R735.9[医药卫生—肿瘤]