脑膜瘤MMP-29、及TIMP-1 mRNA表达与瘤周水肿的相关性研究  

Correlation of the expressions of matrix metalloproteinases-2,9 and tissue inhibitor of metallo-proteinase-1 mRNA in meningioma:correlation with peritumoral edema

作　　者：杨金焰[1] 郭毅[2] 谭艳丽[3] 田春辉[2] 方川[2] 史彦芳[2] 王佳良[2] 韩颖[2] 

机构地区：[1]河北保定市第一医院,保定071000 [2]河北大学附属医院,保定071002 [3]河北大学医学部,保定071002

出　　处：《中国实用神经疾病杂志》2008年第7期37-39,共3页Chinese Journal of Practical Nervous Diseases

摘　　要：目的研究脑膜瘤中基质金属蛋白酶2、9(MMP-2、9)及其抑制因子TIMP-1mRNA表达与其瘤周水肿(PTE)的相关性。方法36例经MRI检查及手术、病理证实的脑膜瘤,根据PTE程度分为2组:无或轻度水肿组和中重度水肿组,将手术标本以免疫组化染色和原位杂交方法对MMP-2、9和TIMP-1 mRNA表达行半定量检测,分析与脑膜瘤瘤周水肿之间的相关性。结果脑膜瘤的瘤周水肿程度与MMP2(P<0.01)和MMP-9(P<0.005)的表达强度差异有统计学意义。而TIMP-1 mRNA的表达在无或轻度水肿组和中重度水肿组之间无显著性差异(P>0.05)。结论MMP-2、9的表达及MMP/TIMP比例失衡在脑膜瘤的高侵袭性和瘤周水肿程度的发生中起关键作用,PTE作为脑膜瘤MRI影像的一个重要特征,因反映了肿瘤的侵袭程度,故可作为临床预后评价的指标之一。Objective To determine whether the expressions of matrix metalloproteinases-2,9（MMP-2,9） and tissue inhibitor of metalloproteinase-1 （TIMP-1） mRNA were correlated with peritumoral edema（PTE） in human meningioma. Methods Thirty-six meningioma specimens were collected for retrospective analysis, which were testified by MRI and proved by operation and pathology. According to the degrees of PTE, the patients were divided 2 groups： one with minimum to mild edema and the other with moderate to mild edema. Protein expressions of MMP-2,9 and mRNA expression of TIMP-1 were semi-quantified in tissue sections by immunohistochemical techniques and in situ hybridization,the results of staining were compared with the degrees of PTE. Results A statistical significance was apparent between the expressions of MMP-2 （P〈0.05）, MMP-9 （P〈0. 05）and peritumor edema（PTE） of meningioma, and there was no statistical significance between the expression of TIMP-1 mRNA and the degree of PTE. Conclusion MMP-2,9 as well as MMPs/TIMP ratio may play an important role in the invasiveness of meningioma and PTE degree. PTE may predict the prognosis of meningiomas.

关 键 词：脑膜瘤 磁共振波谱学 瘤周水肿 明胶酶 基质金属蛋白酶组织抑制因子 

分 类 号：R739.45[医药卫生—肿瘤]