液相色谱-串联质谱法检测水产品中硝基呋喃类代谢物  

Determination of metabolites of nitrofuran antibiotics in aquatic products by high-performance liquid chromatography-tandem mass spectrometry

作　　者：王璟[1] 杨楠[2] 赵晶[1] 权伍英[1] 杨军[3] 

机构地区：[1]沈阳市疾病预防控制中心,沈阳110031 [2]沈阳市卫生监督所,沈阳110014 [3]中国医科大学,沈阳110005

出　　处：《中国卫生检验杂志》2008年第6期978-980,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的：建立硝基呋喃类药物（呋喃唑酮、呋喃它酮、呋喃西林和呋喃妥因）代谢物的液相色谱-串联质谱分析方法。方法：硝基呋喃类代谢物经过二硝基苯甲醛衍生化,用乙酸乙酯提取后经液相色谱分离,电喷雾串联质谱法进行检测。结果：4种硝基呋喃代谢物的定量线性范围均为1～50 ng/ml,线性关系良好（r〉0.99）,水产品中的定量检测限均为0.5μg/kg,回收率在88.0%～94.3%范围内,相对标准偏差在10.4%以下。结论：本法专属性强,灵敏度和准确度高,可作为分析水产品中硝基呋喃类代谢物残留量的有效手段。Objective：To develop a LC - MS/MS method for the simultaneous determination of the metabolites of four nitrofuran antibacterial agents, furazolidone, furaltadone, nitrofurazone and nitrofurantoin in aquatic products. Methods：Samples were derivatised with 2 - nitrobenzaldehyde and extracted by ethyl acetate. The analytes were separated and detected by tandem mass spectrometry with electrospray ionization source. Results： The limits of quantification for four nitrofuran metabolites in samples were 0. 5 μg/kg. The matrix - matched calibration curve showed good linearity （ r^2 〉 0. 99 ） in the range of 1 - 50 ng/ml, The mean recovery was between 88. 0% and 94. 3% and the relative standard derivations less than 10. 4%. Conclusion：This method is specific, sensitive and accurate. It is reliable to determine the nitrofuran metabolites in aquatic products.

关 键 词：液相色谱-串联质谱(LC-MS/MS) 硝基呋喃类代谢物 水产品 

分 类 号：O657.63[理学—分析化学]