气相色谱法同时测定食品中BHA、BHT、TBHQ  

Simultaneous determination of BHA,BHT and TBHQ in foods by gas chromatography

作　　者：叶坚[1] 余涛[1] 

机构地区：[1]广东省肇庆市疾病预防控制中心,广东肇庆526020

出　　处：《中国卫生检验杂志》2008年第6期1082-1083,1127,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的：探讨气相色谱同时测定食品中BHA、BHT、TBHQ的方法。方法：采用甲醇萃取样品,提取液定容后用中性氧化铝吸附杂质,最后于气相色谱仪进行分析定量。结果：BHA、BHT、TBHQ在0～80μg/ml浓度范围内,标准曲线线性关系良好,相关系数r分别为0.9995、0.9997、0.9998;BHA的相对标准偏差为2.6%～5.6%,BHT的相对标准偏差为3.1%～6.8%,TBHQ的相对标准偏差为2.2%～6.5%;BHA的加标回收率为94.4%～102.4%,BHT的加标回收率为94.0%～98.5%,TBHQ的加标回收率为92.4%～101.0%;BHA、BHT检测结果与国标方法比较无显著性差异（P〉0.05）。结论：本方法简便、快捷,适合开展大批量检测工作,而且精密度、准确性均满足检测要求。Objective：To develop a method for the simultaneous determination of BHA, BHT and TBHQ in foods by using gas chromatography. Methods：The BHA, BHT and TBHQ were extracted from samples with methanol, and analyzed by gas chromatography. Results： The linear ranges of determination for BHA, BHT and TBHQ were 0 - 80 μg/ml （ r = 0. 9995 ）, 0 - 80 μg/ml （ r = 0. 9997 ）, 0 - 80 μg/ml （ r = 0. 9998 ）, respectively. The relative standard derivations for BHA, BHT and TBHQ were 2. 6% 5.6% ,3. 1% - 6. 8% , 2. 2%- 6. 5% , respectively. The recoveries of samples for BHA, BHT and TBHQ were 94. 4% 102. 4% ,94. 0% -98. 5% ,92. 4% -101.0%, respectively. Conclusion： The method has the advantages of simplicity, repeatability and sessitivity and can be used for simultaneous analysis of BHA, BHT and TBHQ in foods to protect the health of worker.

关 键 词：叔丁基羟基茴香醚 2 6-二叔丁基羟基甲苯 特丁基对苯二酚 气相色谱 

分 类 号：O657.71[理学—分析化学]