酸性黄分光光度法测定人尿中总蛋白质的研究  

Spectrophotometric determination of proteins in human urine with tropaeolin OO

作　　者：董学芝[1] 邓翠贞[1] 信建豪[1] 焦贺贤[1] 

机构地区：[1]河南大学化学化工学院污染控制研究所,开封475001

出　　处：《药物分析杂志》2008年第6期928-931,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立分光光度法测定人尿中蛋白质含量。方法:利川酸性黄在 pH 1.64的 Clark-Lubs 缓冲溶液中与蛋白质作用形成酸性黄-蛋白质复合物,在532 mm 处测定吸收度。结果:吸收度差值的倒数1/|△A|与蛋白质浓度的倒数(1/c_p)呈良好线性关系,回归方程为1/|△A_|=2.98015+464.1832/c_p ,相关系数为0.9994,线性范围为1.2～360 mg·L^(-1),检出限为0.88 mg·L^(-1)。结论:该法简便、灵敏、准确,测得结果与邻苯二酚红-钼法一致。Objective：To establish a new spectrophotometric method for determination of proteins in human urine. Method ： A complex is formed when protein reacted with tropaeolin OO in Clark - Lubs buffer, and can be quantita- tively determined by spectrophotometric method at 532 nm. Results：There was an excellent linearity between 1/｜ΔA｜ and 1/cp ,the linear equation was 1/｜ΔA｜ =2. 98015 ＋464. 1832/cp,relation coefficient was 0. 9994 in the range of 1.2 -360 mg·L^-1, and the detection limit was 0. 88 mg·L^-1. Conclusion：The method is simple, sensitive and accurate the results obtained by this method agree with those obtained by the pyrogallol red - molybdate complex method.

关 键 词：蛋白质 酸性黄 人尿 

分 类 号：R917[医药卫生—药物分析学]