肝细胞癌患者癌及癌旁组织中FAS及配体的定位  

LOCATION OF FAS AND FAS LIGAND IN HEPATOCELLULAR CARCINOMA AND ITS SURROUNDING TISSUES

作　　者：陈智[1] 刘克洲[1] 章明太[1] 陈明华[1] 何剑琴 茅晓红 

机构地区：[1]浙江医科大学传染病研究所,310003

出　　处：《中华肝脏病杂志》1997年第3期145-146,共2页Chinese Journal of Hepatology

摘　　要：目的:探讨肝细胞凋亡在肝细胞癌发生和发展中所起的作用及其与 HBV 感染的关系。方法:用免疫组织化学方法检测55例肝细胞癌患者的肝癌和癌旁肝组织中的 HBsAg、HBcAg、Fas、FasL,同时用 Tunel 反应原位检测细胞死亡。结果:55例肝癌组织中,41例 HBsAg 阳性,其中25例 HBcAg 阳性;42例可见部分浸润的淋巴细胞中有 Fas 抗原表达,信号较强;癌旁肝组织中 Fas 抗原阳性者23例,肝癌组织中8例,阳性信号均较弱,FasL 在局部浸润的淋巴细胞中检出38例,肝癌及癌旁组织均为阴性。Tunel 反应在3例阳性对照的肝炎标本中呈强阳性反应,肝癌组织中未发现有明显的阳性信号,但有18例标本在浸润的淋巴细胞中检出阳性结果。结论:细胞凋亡机制在 HCC 发生和发展过程中起重要作用。Objective:To explore the effect of hepatocellular apoptosis in occurrence and development of hepatocellular carcinoma and its relationship to HBV infection.Methods:Fas,FasL as well as HBsAg, HBcAg in the hepatocellular carcinoma and its surrounding tissues of 55 HCC patients samples were de- tected by immuno-histochemical method and in situ cell death was also done by Tunel reaction.Results: HBsAg expression was positive in 41 hepatocellular carcinomas of 55 cases,among which 25 cases showed both HBsAg and HBcAg positivity.Partial infiltraling lymphocytes in 42 cases expressed stronger signal of Fas expression,while Fas weak expression in tissues surrounding carcinoma was 23 cases,in cancer tissues only 8 cases.FasL was detected in 38 patients infiltrating lymphocytes but no positive signal was found in hepatocellular carcinomas and their surrounding tissues.TUNEL showed stronger positive reaction in 3 control hepatitis samples and positive reaction in infiltrating lymphocytes of 18 cases but none in liver carcinoma.Conclusion:The mechanism of apoptosis might play an important role in the occurrence and development of HCC.

关 键 词：细胞凋亡 肝肿瘤 癌旁组织 FAS 配体 

分 类 号：R735.702[医药卫生—肿瘤]