营养和环境条件对微生物菌群降解石油的影响  

Effects of nutrition and environment on oil biodegradation by bacterial community

作　　者：李宝明[1] 姜瑞波[1] 

机构地区：[1]中国农业科学院农业资源与农业区划研究所,北京100081

出　　处：《中国土壤与肥料》2008年第3期78-82,共5页Soil and Fertilizer Sciences in China

基　　金：国家科技基础条件平台工作项目(2004DKA30560)支持

摘　　要：从胜利油田分离筛选石油降解菌株并构建了石油降解微生物菌群C9，通过单因素试验确定了菌群C9的最适N源为KNO3，最适P源为K2HPO4，最适pH范围为7～10，最适摇床转数为150～200r／min；正交试验结果表明SL-51、SL-84、SL-133和SL-163的最适接种比例为1：1：20：1，优化后的最佳石油降解条件为：KNO3 3g·L^-1、K2HPO4 1g·L^-1、pH8、总接种量为6．9％，石油浓度为0．25％；气相色谱分析证明，菌群C9在优化后能更有效地降解原油中各种饱和烃和芳烃。Oil degrading bacteria were isolated and bacterial community C9 with oil degrading ability was constructed. KNO3, K2HPO4, pH 7 - 10 and 150 - 200 r/min were determined as the best N source, P source, initial pH values, and rotating speed respectively by sole factor experiment for C9. The orthogonal experiment results showed that the most suitable proportion of strains SL- 51 ：SL- 84：SL- 133：SL- 163 was 1 ： 1：20： 1, and optimum oil degradation condition was KNO3 3 g·L^-1, K2HPO4 1 g·L^-1, and pH 8 respectively, at the same time initial inoculation volume was 6.9%, oil concentrate was 0.25%. Gas chromatography analysis proved that C9 could degrade saturated hydrocarbon and aromatic hydrocarbon more effectively in the optimum conditions.

关 键 词：石油 石油降解菌 菌群 降解率 降解条件 

分 类 号：X172[环境科学与工程—环境科学]