吡格列酮对肥胖小鼠脂肪细胞因子脂联素表达的影响  

Effects of Pioglitazone on the adipocytokines in mice with diet-induced obesity

作　　者：谢宇[1] 朱亭[1] 刘娟[1] 钟毅[2] 丁国宪[1] 

机构地区：[1]南京医科大学第一附属医院老年医学科,江苏南京210029 [2]中国药科大学药化研究室．江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2008年第7期872-875,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金资助项目(NO30570878)

摘　　要：目的:通过构建饮食诱导肥胖小鼠模型,观察吡格列酮对脂肪细胞因子脂联素表达的影响,探讨过氧化物酶体增殖物激活受体(PPAR)-γ激动剂在胰岛素抵抗及2型糖尿病中的作用机制。方法:对肥胖组小鼠予吡格列酮(每日10mg/kg)灌胃14天后处死,放免法测小鼠空腹胰岛素水平,生化法检测血糖,ELISA法检测血清脂联素含量,实时定量PCR检测脂肪组织脂联素的mRNA表达。结果:肥胖组小鼠体重,空腹胰岛素及血糖水平明显升高(P<0.05)。给药后,肥胖干预组小鼠空腹胰岛素降低(P<0.05),血糖降低(P<0.01),血清脂联素含量升高(P<0.01),HE染色显示脂肪细胞体积明显缩小,脂肪组织脂联素mRNA表达升高(P<0.05)。结论:PPAR-γ激动剂通过影响脂肪细胞因子的表达,从而改善胰岛素抵抗。Objective：To investigate the mechanism of Pioglitazone（PGZ） on insulin resistance and type 2 diabetes mellitus by using the DIO（diet-induced obese） mice model. Methods：The C57BL/6J mice were randomly divided into the normal diet group and highfat diet （HFD） group. After 20 weeks,the obese mice were randomly divided into obese control group,PGZ group. They were orally administered placebo and PGZ[ 10 mg/（kg·d）] respectively for two weeks. The adiponectin mRNA expression levels from adipose tissue were analyzed by real-time quantitative PCR. The levels of plasma glucose,serum insulin and adiponectin were measured by Biochemistry technology,Radioimmunity and ELISA. The adipocyte sizes were also observed by Immunohistochemistry. Results：The weight,plasma glucose and serum insulin levels increased （P 〈 0.05） in HFD group,and the adipocyte sizes were bigger. Comparing to obese controls,serum insulin and glucose levels significantly decreased（P 〈 0.05） and the adipocyte sizes reduced in PGZ treatment group, while plasma adiponectin level raised （P 〈 0.01 ）. Moreover,the mRNA expressions of adiponectin upregulated （P 〈 0.05）. Conclusion： The effects of PPAR-γ agonist on insulin resistance can be carried out through regulating the expression of adipocytokines.

关 键 词：胰岛素抵抗 吡格列酮 PPAR-Γ激动剂 脂联素 

分 类 号：Q786[生物学—分子生物学]