Bortezomib或联合三尖杉酯碱、三氧化二砷对急性髓系白血病原代细胞增殖作用的影响  

Study of bortezomib alone or in combination with harringtonine or arsenic trioxide on the proliferation of acute leukemia primary cells

作　　者：蔡艳霞[1] 孟凡义[1] 孙启鑫[1] 扶云碧[1] 李利[1] 

机构地区：[1]南方医科大学南方医院血液科,广州市510515

出　　处：《实用医学杂志》2008年第12期2026-2028,共3页The Journal of Practical Medicine

基　　金：广东省科技计划项目(编号:2007B031515005)

摘　　要：目的:探讨bortezomib单独或联合三尖杉酯碱(HT)、三氧化二砷(As2O3)对初发及难治/复发急性白血病原代细胞的增殖作用。方法:以初发及难治/复发急性髓系白血病原代细胞为研究对象,分别应用bortezomib、HT、As2O3单独或联合处理细胞后,使用MTT法观察细胞增殖活力。结果:10～500nmol/L bortezomib对初发及难治/复发性白血病患者原代细胞均有抑制作用,且呈明显剂量依赖关系,随浓度增大抑制效果逐渐增强。作用于初发白血病原代细胞时,Bortezomib与HT联合与各单药处理组相比,抑制率无明显提高,而联合As2O3时其抑制率优于单药处理组;作用于难治/复发白血病原代细胞时,bortezomib与HT或As2O3联合效果均呈现相加作用,其中与As2O3联合效果优于与HT联合者。结论:Bortezomib能够抑制初发及难治/复发急性白血病原代细胞增殖,与As2O3联合作用后抑制效果明显增强。Objective To investigate the effect of bortezomib alone or combined with harringtonine （HT） or arsenic trioxide （As2O3） on the proliferation activity of the de novo and the refractory/relapse acute leukemia primary cells. Methods The de novo and the refractory/relapse acute leukemia primary ceils were incubated with bortezomib at different doses alone or in combination with HT or As2O3. The proliferation activity was observed by MTT assay. Results In bortezomib-treated tumor cells, inhibition rate enhanced in dose-dependent manner. For the de novo acute leukemia primary cells, the inhibit rate of bortezomib combined with HT was not significant different from those of either of the compounds above, whereas the inhibit rate of bortezomib combined with As2O3 was enhanced compared with those of either of the compounds above. For the refractory/relapse acute leukemia primary cells, the effects of bortezomib combined with HT or As2O3 were much better than those of either of the compounds above, moreover, the effect of bortezomib in combination with As2O3 was better than that of bortezomib in combination with HT. Conclusion Bortezomib can inhibit proliferations of the de novo or refactory/relapse acute leukemia primary cells and appear to have additive effect when combined with As2O3.

关 键 词：白血病 三尖杉酯碱 BORTEZOMIB 三氧化二砷 

分 类 号：R733.7[医药卫生—肿瘤]