Detection of microarray protein biomolecules by oblique-incidence reflectivity difference technique without labelling agents  

作　　者：张洪艳 陆珩 李巍 梁汝强 金奎娟 周岳亮 阮康成 杨国桢 

机构地区：[1]Laboratory of Optical Physics, Institute of Physics and Center for Condensed Matter Physics, Chinese Academy of Sciences [2]Laboratory of Organic Optoelectronic Functional Materials and Molecular Engineering, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences [3]Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences

出　　处：《Chinese Physics B》2008年第6期2288-2291,共4页中国物理B（英文版）

基　　金：Project supported by the Chinese Academy of Sciences for Key Topics in Innovation Engineering (Grant No L3FX051W)

摘　　要：This paper reports that the detection to the protein in microarray format is carried out by oblique-incidence reflectivity difference （OI-RD） analysis without any labelling agents. The OI-RD intensities not only depend on the protein structure, but also vary with the protein concentration. The results indicate that this method should have potential application in detection of biochemical processes. The high throughout and in situ detection can be achieved by this method with further improving of the experimental system.This paper reports that the detection to the protein in microarray format is carried out by oblique-incidence reflectivity difference （OI-RD） analysis without any labelling agents. The OI-RD intensities not only depend on the protein structure, but also vary with the protein concentration. The results indicate that this method should have potential application in detection of biochemical processes. The high throughout and in situ detection can be achieved by this method with further improving of the experimental system.

关 键 词：DETECTION OI-RD PROTEIN MICROARRAY 

分 类 号：Q51[生物学—生物化学] TN409[电子电信—微电子学与固体电子学]