甘氨酸脂质体对心肌细胞线粒体膜电位及凋亡的影响  

Effect of glycine liposomes on mitochondrial membrane potential and apoptosis in cultured cardiomyocytes

作　　者：陈梦飞[1] 陆大祥[1] 戚仁斌[1] 王华东[1] 王彦平[1] 张俊艳[1] 李楚杰[1] 

机构地区：[1]暨南大学医学院病理生理教研室

出　　处：《中国病理生理杂志》2008年第7期1254-1258,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30470718);广东省自然科学基金资助项目(No.04105844);广州市科技局重点资助项目(No.2004Z3-E4081)

摘　　要：目的:观察甘氨酸脂质体对缺氧/复氧损伤心肌细胞线粒体膜电位及凋亡的影响。方法:建立培养乳鼠心肌细胞缺氧/复氧(H/R)损伤模型,以DiOC6(3)为荧光分子探针检测实验各组心肌细胞线粒体膜电位;以AnnexinV联合PI染色法检测实验各组心肌细胞凋亡率。结果:(1)H/R处理组心肌细胞线粒体膜电位明显低于对照组(P<0.01),甘氨酸脂质体处理组心肌细胞线粒体膜电位降低最少,弱荧光部分细胞百分率为(9.61±0.76)%。与甘氨酸组比较有显著差异(P<0.01)。(2)H/R处理组心肌细胞凋亡率为(20.78±1.58)%,明显高于对照组(P<0.01)。甘氨酸脂质体处理组心肌细胞凋亡发生率低于甘氨酸组(P<0.01)。空白脂质体组细胞凋亡发生率与H/R组比较差异无统计学意义(P>0.05)。结论:甘氨酸脂质体能抑制培养心肌细胞缺氧/复氧损伤诱导的心肌细胞线粒体膜电位下降和心肌细胞凋亡,脂质体携载甘氨酸能更好发挥其细胞保护作用。AIM： To observe the effect of glycine liposomes on the mitochondriai membrane potential and the apoptosis rate in cardiomyocytes induced by hypoxia/reoxygenation injury. METHODS： A cardiomyocyte injury model was established by using hypoxia/reoxygenation. DiOC6 （3） as fluorescence molecular probe was used to detect the mitochondri- al membrane potential in each group. The method of Annexin V associated with PI was used to detect the apoptosis ratio in each group. RESULTS： （ 1 ） The result of flow cytometry showed that the mitochondrial membrane potential of cardiomyo- cytes in H/R group was obviously lower than that in control group （P 〈 0.01 ）. The decrease in mitochondrial membrane potential in Gly - liposome group was the lowest, the percentage of cells about the part of hypofluorescence was （9. 61 ± 0. 76 ） %, which was lower than that in glycine group （ P 〈 0. 01 ）. （2） The apoptosis rate of cardiomyocytes in I-I/R group was higher than that in control group （20. 78 ± 1.58）% ,P 〈0. 01. After the treatment of Gly - liposome, the apoptosis rate of cardiomyocytes was lower than that in glycine group （P 〈 0. 01 ）. No difference in the apoptosis ratios between blank - liposome group and H/R group was observed （ P 〉 0. 05 ）. CONCLUSION ： Glycine liposomes protect cultured cardiomyocytes against hypoxia/reoxygenation injury. Glycine liposomes produce the better protective effects than glycine.

关 键 词：甘氨酸 脂质体 心肌细胞 心肌再灌注损伤 细胞凋亡 线粒体膜电位 

分 类 号：R363[医药卫生—病理学]