血管内皮生长因子受体-2在植入前小鼠胚胎发育中的表达变化  

Expression changes of vascular endothelial growth factor receptor-2 in mouse preimplantation embryos

作　　者：韩雅玲[1] 李颖佳[1] 齐岩梅[1] 康建[1] 闫承慧[1] 

机构地区：[1]沈阳军区总医院全军心血管病研究所心内科,辽宁沈阳110016

出　　处：《中国病理生理杂志》2008年第7期1259-1262,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30370526)

摘　　要：目的:探讨植入前小鼠胚胎血管内皮生长因子受体-2(VEGFR-2/Flk-1)的表达规律及其在小鼠胚胎发育中的作用。方法:对0.5-3.5d小鼠胚胎行全胚免疫荧光染色及反转录-聚合酶链反应,观察胚胎发育过程中Flk-1表达时相及其变化;取8细胞期胚胎行体外培养,加入5mg/LFlk-1中和抗体,分析36h囊胚形成率。结果:在植入前小鼠胚胎中Flk-1mRNA于4细胞期开始表达,8细胞期达高峰,桑椹胚期和囊胚期无表达;Flk-1蛋白表达定位于胚胎外缘和细胞交界处,于4细胞期和8细胞期表达呈阳性,桑椹胚期、囊胚期表达呈阴性;于体外培养的8细胞期培养液中加入5mg/L中和抗体后,36h囊胚形成率降低。结论:在小鼠植入前胚胎发育不同阶段Flk-1表达不尽相同,其作用可能与Flk-1促进植入前胚胎发育有关。AIM ： To investigate the expression pattern and role of vascular endothelial growth factor receptor - 2 （ VEGFR - 2/Flk - 1 ） in mouse preimplantation embryos. METHODS ：The expression changes of Flk - 1 in 0.5 to 3.5 - day mouse embryos were detected by using whole - mount immunofluorescence on a laser - scanning confocal microscopy and by reverse transcription polymerase chain reaction. Following culture of 8 - cell embryos in medium with 5 mg/L Flk - 1 neutralization antibody, blastula formation ratio was analyzed. RESULTS ： Flk - 1 mRNA was first detected in the 4 - cell embryos, and most abundant in the 8 - cell embryos, but was not detected in the morula and blastula. The protein expression of Flk - 1 was localized to outer margin and cell boundary of the 4 - cell embryos and 8 - cell embryos, not the morula and blastula embryos. When the 8 - cell embryos were cultured in medium with 5 mg/L Flk - 1 neutralization anti- body, the blastula formation ratio at 36 h was lower than that without Flk - 1 neutralization antibody. CONCLUSION ： The expression of Flk - 1 varies at different stages of the preimplantation embryos, the function of which may be involved in the development of preimplantation embryos.

关 键 词：受体 血管内皮生长因子 胚胎发育 小鼠 

分 类 号：R363[医药卫生—病理学]