冠心病与蛋白Z内含子FG79A基因多态性无关联  

Protein Z intron FG79A polymorphisms are not associated with coronary artery disease

作　　者：邵红[1] 徐耕[2] 吴立萱[1] 屈百鸣[1] 

机构地区：[1]浙江省人民医院心内科,浙江杭州310014 [2]浙江大学医学院附属第二医院心内科,浙江杭州310009

出　　处：《中国病理生理杂志》2008年第7期1270-1274,共5页Chinese Journal of Pathophysiology

基　　金：浙江省科技厅重点攻关资助项目(No.021103166)

摘　　要：目的:探讨中国浙江地区汉族人群中蛋白Z(protein Z,vitamin K-dependent plasma glycoprotein,PROZ)内含子FG79A基因多态性的发生率及其与冠心病的关系。方法:对冠状动脉粥样硬化性心脏病(简称冠心病)组(至少1支冠脉动脉直径狭窄≥50%)148例和147例对照者采用聚合酶链反应和限制性内切酶片段长度多态性方法结合基因测序技术,检测蛋白Z内含子FG79A基因多态性。结果:首次发现中国浙江地区汉族人群存在蛋白Z内含子FG79A基因多态性,野生型(GG型)、GA型和AA型分别占16.95%、54.58%和28.47%;G、A等位基因频率分别为44.24%和55.76%;冠心病组与对照组间基因型和等位基因频率分布差异均无显著性意义(均P>0.05);冠脉单支、双支和三支病变患者间基因多态性分布差异无显著性意义(P>0.05),蛋白Z内含子FG79A基因型和等位基因频率在急性冠脉综合征和非急性冠脉综合征患者间的分布差异亦无显著性意义(均P>0.05)。结论:中国浙江地区汉族人群存在蛋白Z内含子FG79A基因多态性,但蛋白Z内含子FG79A基因多态性与冠心病及急性冠脉综合症的发生无关。AIM： To investigate the distribution of protein Z intron FG79A polymorphisms in Chinese and the associations with coronary artery disease （CAD）. METHODS： 148 patients were performed selected coronary angiography and more than one major coronary vessel with at least 50% stenosis was defined as CAD. The control group consisted of 147 subjects. The protein Z intron FG79A polymorphisms were studied by polymerase chain reaction and restriction fragment length polymorphism and parts of PCR products were sequenced. RESULTS ： Protein Z intron FG79A polymorphisms were first recovered in Chinese, and the frequencies of G and A alleles were 44. 24% and 55.76%, respectively. The fre- quencies of two alleles were not significantly different between patients and controls. There was no significant difference in protein Z intron FG79A genotype distribution among patients with one, two or three stenosed vessels. No significant difference was found among the frequencies of the three genotypes between both acute coronary syndrome （ACS） and non - ACS. CONCLUSION： Protein Z intron FG79A polymorphisms are present in Chinese. Protein Z intron FG79A polymorphisms were not associated with CAD and ACS.

关 键 词：冠状动脉疾病 蛋白质Z 基因多态性 中国汉族人群 

分 类 号：R363[医药卫生—病理学]