缺血后处理减轻大鼠骨骼肌缺血再灌注损伤及其微循环机制研究  

Ischemic postconditioning protects skeletal muscle from ischemia/reperfusion injury through improving microcirculation in rat hind limb

作　　者：张振英[1] 刘秀华[1] 郭晓笋[1] 刘凤英[1] 胡维诚[2] 

机构地区：[1]中国人民解放军总医院病理生理研究室,北京100853 [2]山东大学医学院病理生理学教研室,山东济南250012

出　　处：《中国病理生理杂志》2008年第7期1404-1409,共6页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30670822);国家自然科学基金重大国际合作资助项目(No.30620130111)

摘　　要：目的:观察缺血后处理(I-postC)对大鼠后肢骨骼肌缺血再灌注(I/R)损伤的影响,并探讨其微循环机制。方法:健康雄性Wistar大鼠48只,随机分为I/R组、I-post C组和预处理(IPC)组(n=16),无创动脉夹夹闭股动脉4h,松夹再灌注12h或24h建立大鼠后肢I/R模型。于再灌注结束时分别检测大鼠胫前肌血氧饱和度(StO2)、血流量、微循环以及血流动力学变化,并测定骨骼肌湿干重比值(W/D),观察骨骼肌超微结构变化。结果:(1)I-postC12h组W/D较I/R12h组低6.7%(P<0.05),骨骼肌肌原纤维水肿、溶解、分离和线粒体空泡样变性显著减轻;再灌注24hI-postC组左室压最大下降速率(-dp/dtmax)较I/R24h组高10.46%(P<0.05),与IPC组无显著差异。(2)再灌注24hI-postC组实验侧细静脉血流速度较I/R组高11.3%(P<0.05),细动、静脉收缩减轻(细动、静脉内径较I/R组分别高39.8%和24.1%,P<0.01),与IPC的保护效果接近(P>0.05)。结论:I-postC显著减轻骨骼肌I/R损伤,其保护机制与改善微循环有关。AIM： To investigate the effect of ischemic postconditioning （ I - postC） on ischemia/reperfusion （I/R） injury of skeletal muscle and its microvasculatory mechanisms in rat hind limb. METHODS： Healthy male Wistar rats were randomly divided into three groups as follows （n = 16 ） ： I/R group, I -postC group and ischemic preconditioning （IPC） group. I/R of fight hind limb was induced by clamping right femoral artery. The effects of I -postC on oxygen saturation of hemoglobin （St02）, blood flow, microcirculation, hemodynamics, Wet/Dry ratio （W/D） and ultramicrostructure of skeletal muscle were detected on 12 h or 24 h after reperfusion. RESULTS ： （ 1 ） Compared with I/R group, 12 h after reperfusion, W/D from I - postC group decreased 6. 7 % （P 〈 0. 05 ） and myofibrillae lysis of skeletal muscle and vacuola- tion of mitochondria attenuated than that in I/R group. 24 h after reperfusion, - dp/dtmax in I - postC group increased 10. 46% （P 〈 0. 05 ）. （2） It was also found that 24 h after reperfusion, blood velocity in venule increased 11.3% in I - poatC group compared with that in I/R group （P 〈0. 05）, and internal diameter of arteriole and venule increased 39. 8% and 24. 1% compared with that in I/R group, respectively （P 〈0. 01 ）. No significant difference between I - postC and IPC groups （P 〉 0. 05 ） was found. CONCLUSION： I -postC protects the skeletal muscle against reperfusion injury by improving microcirculation.

关 键 词：再灌注损伤 缺血后处理 肌  骨骼 微循环 

分 类 号：R363[医药卫生—病理学]