白细胞介素-18干预诱导的树突状细胞表型和活性研究  

Phenotype and immune activity of dendritic cells under interleukin-18 intervention

作　　者：傅永强[1] 张在云[2] 李旭升[1] 柳月安[3] 

机构地区：[1]金华职业技术学院医学院,浙江金华321017 [2]山东大学第二医院肿瘤科 [3]山东省肿瘤医院药剂科,山东济南250033

出　　处：《中国病理生理杂志》2008年第7期1414-1417,共4页Chinese Journal of Pathophysiology

基　　金：金华市科技创新重点资助项目(No.2004-2-302)

摘　　要：目的:研究白细胞介素-18(IL-18)干预诱导的树突状细胞(DC)的表型和活性。方法:自人外周血单核细胞诱导DC,第5d起分为IL-18组、TNF-α组和IL-18+TNF-α组,分别加IL-18、TNF-α及IL-18+TNF-α促成熟,用ELISA法测定上清中IL-12含量;用流式细胞仪测定培养8dDC的CD1a、HLA-DR、CD83及CD86的表达;用MTT法检测3组DC诱导T细胞增殖的作用。用ELISA法测定3组DC刺激T细胞分泌干扰素γ(IFN-γ)的量。结果:IL-18组与TNF-α组CD1a、HLA-DR、CD83及CD86表达无差异,IL-18+TNF-α组CD1a、CD83及HLA-DR阳性率高于IL-18组。IL-18+TNF-α组IL-12量高于IL-18组和TNF-α组(P<0.05)。IL-18组与TNF-α组DC刺激T细胞增殖作用无差异,IL-18+TNF-α组DC的作用强于IL-18组和TNF-α组。IL-18组和TNF-α组IFN-γ量无显著差异,IL-18+TNF-α组IFN-γ的量高于IL-18组和TNF-α组(P<0.05)。结论:IL-18干预诱导的DC高表达表面分子,具有明显的免疫刺激活性,IL-18与TNF-α合用作用更强。AIM： To investigate the phenotype and immune activity of dendritic cells using interleukin - 18 as intervent. METHODS： Monocytes were isolated from human peripheral blood and induced into DCs with GM - CSF and IL -4. The cellular morphous was Observed under inverted microscope. On the 5th day, 3 groups including IL - 18 group, TNF - a group and IL - 18 ＋ TNF -α group were set. IL - 18, TNF -α or IL - 18 ＋ TNF -α was used as intervents respectively to facilitate cell maturity. Supernatants were collected at 24 h, 48 h and 72 h. IL - 12 in the supematant, CDla, HLA - DR, CD83 and CD86 were analyzed using flow cytometry. DCs of the 3 groups were co - cultured with T cells respectively on the ratio of 1： 100, 1：50 and 1： 10. T cell proliferation stimulated by DC was determined using MTr method. DCs were co - cultured with T cells on the ratio of 1： 10, and the supernatant were collected at 24 h, 48 h and 72 h. IFN - γ in the supernatant was detected with ELISA method. RESULTS： Induced by GM - CSF and IL - 4, then stimu- lated by IL- 18, TNF-α or IL- 18 ＋ TNF-α, monocytes showed typical morphous of DC. No morphological difference was observed among DCs of the 3 groups. No statistical difference showed in expression level of CDla, HLA - DR, CD83 and CD86 between IL - 18 group and TNF-α group （P 〉0. 05）. The positive rates of CDla and CD83 in IL - 18 ＋TNF -α group were higher than those in other 2 groups. The positive rate of HLA - DR in IL - 18 ＋ TNF -α group was higher than that in IL -18 group. No difference between IL - 18 group and TNF-α group in the potency of stimulating T cell proliferation was found, whereas the stimulating potency in IL - 18 ＋ TNF-α group was higher than that in IL - 18 group and TNF -α group. IL - 12 in IL - 18 ＋ TNF -α group at 48 h and 72 h was higher than that in IL - 18 group and TNF - 0t group （P〈0.05）. However, there was no difference between the latter 2 groups. There was also no difference between IL- 18 group and TNF -ct group in I

关 键 词：白细胞介素18 树突细胞 干预性研究 

分 类 号：R363[医药卫生—病理学]