甲状腺乳头状癌组织中钠碘转运体基因启动子甲基化的研究  

Study on methylation of sodium iodide symporter gene promoter in papillary thyroid carcinoma

作　　者：唐建东[1] 栗夏莲[1] 杜潇潇[1] 邱新光[2] 张全武[3] 

机构地区：[1]郑州大学第一附属医院内分泌科,450052 [2]郑州大学第一附属医院甲状腺外科 [3]郑州市中心医院病理科

出　　处：《中华内分泌代谢杂志》2007年第6期508-509,共2页Chinese Journal of Endocrinology and Metabolism

基　　金：河南省科委自然科学基金(0311041600)

摘　　要：用甲基化特异性PCR检测34例甲状腺乳头状癌及癌旁组织中钠碘转运体(NIS)基因启动子的甲基化。结果显示癌组织中NIS基因启动子甲基化显著高于癌旁组织(P<0.05),淋巴结转移组中NIS基因启动子的甲基化显著高于无淋巴结转移组(P<0.05),提示NIS基因启动子异常甲基化是甲状腺乳头状癌发展过程中常见的分子事件,可能影响了甲状腺乳头状癌细胞的摄碘功能。The methylation-specific PCR technique was applied to detect the promoter methylation of sodium iodide symporter （NIS） gene in 34 cases of papillary thyroid carcinoma （PTC） and adjacent thyroid tissure （ATF）. The results showed that the methylation of NIS promoter in PTC was significantly higher than that in ATF （ P 〈 0.05 ）. The methylation in patients with lymph node metastasis was also significantly higher than that without lymph node metastasis （ P 〈 0.05 ）, suggesting that the methylation of promoter region in NIS gene is a common molecular event and may play a role in iodide-uptake of human PTG.

关 键 词：甲状腺乳头状癌 基因 钠碘转运体 DNA甲基化 甲基化特异性PCR 

分 类 号：R736.1[医药卫生—肿瘤]