灯盏花素对人脐静脉内皮细胞增殖及基质金属蛋白酶-2的影响  

Influence of Breviscapine on the Proliferation and Thrombin-induced Expression of Matrix Metalloproteinase-2 in Cultured Human Umbilical Vein Endothelial Cell

作　　者：冯秀元[1] 李鸣媛[2] 谭维羚[2] 沈江山[2] 

机构地区：[1]南昌大学研究生院医学部 [2]南昌大学第四附属医院心内科,南昌330006

出　　处：《江西医学院学报》2008年第3期9-11,15,F0002,共5页Acta Academiae Medicinae Jiangxi

基　　金：江西省卫生厅中医药科研基金课题(2007A014)

摘　　要：目的研究灯盏花素对人脐静脉内皮细胞(HUVEC)生长的影响及不同浓度的灯盏花素对凝血酶诱导的HUVEC基质金属蛋白酶-2(MMP-2)表达的影响。方法用MTT比色法观察灯盏花素作用48h后对HUVEC增殖的影响,用培养细胞片免疫细胞化学染色的方法来评价不同浓度的灯盏花素(17.50、35.00、70.00mg/L)对凝血酶诱导HUVEC分泌MMP-2表达的情况。结果灯盏花素在浓度≤78.13mg/L时,对HUVEC的增殖无影响(P>0.05),而在浓度为156.25、312.50、625.00、1250.00mg/L时明显抑制细胞增殖(P<0.01),其作用的IC50为226.90mg/L;与凝血酶组相比,灯盏花素各浓度组均能抑制凝血酶诱导的HUVEC分泌MMP-2(P<0.01),并呈浓度依赖性。结论高浓度灯盏花素对体外培养HUVEC的增殖有抑制作用;灯盏花素能以浓度依赖性的形式抑制凝血酶诱导的体外培养的HUVEC分泌MMP-2的表达;可能对抗动脉粥样硬化、稳定硬化斑块、防治斑块破裂和预防急性冠状动脉综合征的发生产生有益的作用。Objective To investigate the effects of breviscapine at different concentrations on the proliferation and expression of matrix metalloproteinase-2（MMP-2） induced by thrombin in cultured human umbilical vein endothelial celIs（HUVEC）. Methods The effects of brevescapine on the proliferation of HUVEC was observed after 48 hours by MTT assay in vitro. The breviscapine of different concentrations（17. 50,35. 00 and 70.00 mg/L）were conincubated with cultured HUVEC for 24 hours after the HUVEC were stimulated in 1 hour by thrombin（4 kU/L） ,Expression of MMP-2 were determined by immunocytochemistry. Rsesuits Compared with normal control group after 24 hours, breviscapine group （478.13 mg/L） had no obvious effect on the proliferation of cultured HUVEC（P〉0. 05）, but 156. 25 mg/L, 312. 50 mg/L, 625. 00 rag/L, 1 250.00 mg/L breviscapin inhibited the proliferation of cultured HUVEC（P〈0.01）with IC50 = 226.90 mg/L. Breviscapine dose-dependently inhibited the expression of MMP-2 induced by thrombin. Conclusion Breviscapine could inhibit the proliferation of the expression of MMP-2 in cultured HUVEC. P, reviscapine could decrease the expression of MMP-2 in thrombin-induced HUVEC,which would be beneficial for the anti-atherosclerosis, atherosclerotic plaque stability,the prevention of plaque rupture and the occurrence of acute coronary syndrome.

关 键 词：凝血酶 基质金属蛋白酶-2 灯盏花素 人脐静脉内皮细胞 

分 类 号：R-33[医药卫生] R349.5