KIO_4-二溴硝基偶氮胂体系阻抑动力学光度法测定痕量EDTA  

Determination of Trace EDTA by Inhibition Kinetic-spectrophotometry in KIO4-Dibromo-nitro Arsenazo System

作　　者：杨杰 于惠春 

机构地区：[1]山东省蓬莱市卫生防疫站,山东蓬莱265600 [2]烟台张裕葡萄酿酒股份有限公司,山东烟台264000

出　　处：《预防医学论坛》2008年第8期744-746,共3页Preventive Medicine Tribune

摘　　要：[目的]研究阻抑动力学光度法测定痕量EDTA的新体系。[方法]在硫酸介质中，Fe（Ⅲ）催化KIO4氧化二溴硝基偶氮胂的反应可被微量EDTA有效地阻抑，被过量EDTA有效的终止，据此建立了测定痕量EDTA的动力学光度法，用固定时间法在510nm波长处监测阻抑反应。[结果]方法的线性关系良好，线性范围为0～50．0μg／50ml，检出限为2．0×10^-6 mg／mL，标准加入回收率为97．0％～102．0％。[结论]该方法用于罐头等食品中EDTA的测定，获得满意结果。[Objective]To study a new system of determining trace EDTA by inhibition kinetic- speetrophotometry. [Methods]In the medium of H2SO4 solution, the catalytic action of Fe（Ⅲ）on the oxidation of Dibromo-nitro Arsenazo by KIO4 was effectively inhibited by EDTA. The reaction was effectively stopped by superfluous EDTA. The decrease in absorbanee was measured at 510 nm at fixed time intervals. [Resuits]The linear determination range is between 0 and 50.0 μg/50 ml of EDTA. A detection limit of 2.0×10^-6 mg/mL was achieved. The recoveries were 97.0%-102.0%. [Conclusion]The method can be applied in the determination of trace EDTA in canned food with satisfactory results.

关 键 词：动力学光度法 EDTA 二溴硝基偶氮胂 KIO4 

分 类 号：R155.5[医药卫生—营养与食品卫生学]