原发性膀胱移行细胞癌 D17S5 VNTR 位点的遗传多态性研究  

D17S5 LOCI VNTR GENETIC POLYMORPHIC STUDY IN PRIMARY BLADDER TRANSITIONAL CELL CANCER

作　　者：孔令斌[1,2,3] 魏琦 王德育[1,2,3] 马玉莲 杨景玉[1,2,3] 

机构地区：[1]济宁医学院附属医院 [2]济宁市中医院 [3]西安医科大学

出　　处：《济宁医学院学报》1997年第2期4-6,共3页Journal of Jining Medical University

基　　金：卫生部青年科学基金

摘　　要：Ｄ１７Ｓ５ＶＮＴＲ定位于人类第１７号染色体的短臂１区３带，与Ｐ５３基因紧密连锁，在Ｍｉｌｅｒ—Ｄｉｃｋｅｒ综合征中发现有Ｄ１７Ｓ５ＶＮＴＲ的信息丢失。为了进一步了解此基因的功能，本文采用聚合酶链反应结合聚丙烯酰胺凝胶电泳的方法对膀胱移行细胞癌的Ｄ１７Ｓ５ＶＮＴＲ的遗传多态性进行了研究。结果显示：膀胱移行细胞癌中Ｄ１７Ｓ５ＶＮＴＲ位点的频率不符合Ｈａｒｄｙ—Ｗｅｉｂｅｒｇ平衡；且ＰＹＮＺ２２ＶＮＴＲ位点的频率分布与正常人群之间存在显著性差异，肿瘤中纯合子的频率高于正常人群。结果表明：Ｄ１７Ｓ５ＶＮＴＲ可作为膀胱癌基因诊断的一个有用指标。D17S5 vairble number of tandem repeat (VNTR) mapping 17th chrosome,link p53 gene,which is relatde to Miller-Dieker syndrome,using polymerase chain reaction,following high solution PAGE technique, 26 cases of TCC D17S5VNTR are amplified. The results showed that D17S5VNTR loci frequency dose not fit Hardy—Weiberg balance in TCC(P<0 01); and compared with normal controls, the frequency of PYNZ22VNTR shows significant difference; the frequency of hetreogygosity in tumor organism is less than that in normal organism. These results suggest that the charge of D17S5VNTR frequency may be an important sign of the primary bladder transitional cell cancer.

关 键 词：D17S5 VNTR 膀胱肿瘤 遗传多态性 

分 类 号：R737.140.2[医药卫生—肿瘤]