螺杆菌致病的动物实验观察  

Animal experimental study on the pathogenicity of helicobacter

作　　者：王继德[1] 周殿元[1] 张亚历[1] 谢国建[1] 张万岱[1] 杨海涛[1] 

机构地区：[1]第一军医大学南方医院,广州市510515

出　　处：《中华消化杂志》1997年第6期315-317,共3页Chinese Journal of Digestion

摘　　要：目的：在活体动物中观察螺杆菌的致病性。方法：建立猫胃螺杆菌的小鼠感染模型，观察感染不同时间后胃粘膜的病理变化和血清中抗体产生情况。结果：螺杆菌的较长期感染（＞24周）主要引起动物胃粘膜以淋巴细胞浸润或淋巴滤泡形成为主的慢性炎症，且感染时间越长、病变越重，感染40周后可出现胃粘膜糜烂和中性粒细胞浸润等急性炎症改变，部分动物出现嗜酸性粒细胞浸润．血清和胃肠液中产生与Hp有交叉反应的非保护性抗体，但无溃疡发生。结论：螺杆菌感染是宿主慢性胃炎的病因；猫胃螺杆菌动物模型易于建立，与Hp感染人类的病变类似，可以有效地应用于Hp的防治研究。Objective:To study the pathogenicity of Helicobacter in vivo. Method:SPF Balb/cmice were innoculated with Helicobacter fells(Hf). Pathological changes in gastric mucosa ofanimals were observed in varying durations of infection, humoral antibodies to Hf and Helicobacterpylori(Hp) were detected too. Results:Chronic gastritis characterized by infiltration of lymphoidcells and formation of lymphoid follicles could be found after 24 to 40 weeks of infection. Acutegastritis features such as erosion of gastric mucosa and infiltration of neutrophilic cells could befound (40 weeks) afterwards, infiltration of eosinophilic cells could be found sporadically in gastricmucosa of some animals. IgG antibodies with cross reactivity with Hp could be detected in serumand intestinal fluid of mice infected for 40 weeks. Conclusion:There were strong correlationshipbetween the development of chronic gastritis and Hf infection, mouse model infected with Hf mightbe very useful in the study of Hp.

关 键 词：幽门螺杆菌 致病性 猫胃螺杆菌 动物模型 

分 类 号：R573.02[医药卫生—消化系统] R－332[医药卫生—内科学]