利用RNA干扰技术研究14-3-3ζ对肺癌细胞转移的抑制作用  

Inhibition of metastasis of lung cancer cells by 14-3-3ζ:a study on with RNAi technology

作　　者：陈惠华[1] 谭晓刚[1] 王妍[1] 徐元基[1] 杜芝燕[1] 陆应麟[1] 

机构地区：[1]军事医学科学院基础医学研究所,北京100850

出　　处：《军事医学科学院院刊》2008年第3期224-227,共4页Bulletin of the Academy of Military Medical Sciences

基　　金：国家自然科学基金资助(30271452;30471947);国家"973"计划资助项目(2002CB513105)

摘　　要：目的:探讨14-3-3ζ对肺癌细胞转移的押制作用。方法:以人肺巨细胞癌低转移细胞株为模型,利用RNAi技术研究14-3-3ζ被干涉后肿瘤细胞恶性表型的变化,探讨14-3-3ζ与肿瘤转移的相关性。结果:本研究发现14-3-3ζ被干涉后细胞的集落形成能力明显提高,黏附能力明显降低,细胞迁移、侵袭能力明显提高。结论:14-3-3ζ可能抑制肺癌细胞的转移。Objective：To investigate the inhibition of metastasis of lung cancer cells by 14-3-3ζ. Methods： RNAi technology was employed. The RNAi expression vectors of 14-3-3ζ were constructed and transfected into PLA-801C （the poorly metastatic subline of human lung giant cell carcinoma） . The cell cycle alteration, cell＇s ability of proliferation, adhesion, migration and invasion after transfection were studied. Results： Cells transfected with RNAi vector showed much lower 14- 3-3ζ expression and ability of adhesion, but much better ability of proliferation,migration and invasion. Conclusion： 14-3- 3ζ may inhibit the metastasis of lung cancer cells.

关 键 词：14-3-3ζ 肿瘤转移 肺肿瘤 RNA干扰 

分 类 号：R734.2[医药卫生—肿瘤]