DHA对小鼠脂肪组织和肝脏生脂及脂解基因转录表达的影响  

Effect of DHA on transcription expression of lipogenic and lipolytic genes in mice fat tissue and liver

作　　者：魏周文[1] 孙超[1] 姜东凤[1] 侯增淼[1] 刘春伟[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨陵712100

出　　处：《西北农林科技大学学报（自然科学版）》2008年第7期1-8,共8页Journal of Northwest A&F University(Natural Science Edition)

基　　金：教育部重点项目(105167);国家自然科学基金项目(30471267);教育部新世纪优秀人才计划项目(NCET-06-0865)

摘　　要：【目的】探讨二十二碳六烯酸(DHA)对小鼠脂肪组织和肝脏生脂相关基因过氧化物酶增殖物激活受体γ(PPARγ)、固醇调节元件结合蛋白-1c基因(SREBP-1c)、脂肪酸合成酶基因(FAS),及脂解相关基因激素敏感脂酶基因(HSL)和甘油三酯水解酶(TGH)时序表达的影响。【方法】用不同浓度DHA(6.25和12.5 g/kg)灌胃小鼠,分别于0,1,2,4,8,16和24 h处死,取脂肪组织和肝脏,提取总RNA,半定量(semi-quantitative,SQ)RT-PCR方法检测生脂及脂解基因PPARγ、SREBP-1c、FAS,及脂解相关基因HSL和TGH的时序表达规律。【结果】脂肪组织中DHA均可促进PPARγ、FAS、HSL和TGH基因的转录表达,8 h达到峰值,其后表达量下降;抑制SREBP-1c基因的表达,8 h达到最低,之后上升。肝脏中DHA均可抑制PPARγ、SREBP-1c、FAS和HSL基因的转录表达,且表达量分别于8,8,8和16 h达到最低,之后表达量上升;DHA对TGH的作用不显著(P>0.05)。【结论】DHA通过促进脂肪组织中脂肪分解及抑制肝脏中脂肪合成与分解来调节动物体脂沉积。[Objective] The paper discussed how docosahexaenoic acid （DHA）regulated transcription expression of lipogenic and lipolytic genes, which included PPARγ （peroxisome proliferators activated receptor γ）,SREBP-1c （sterol regulatory element binding protein-1c）, FAS （fatty acid synthetase）, HSL （hormone-sensitive lipase） and TGH （triglyceride hydrolyase）. [Method] Mice were fed with different concentrations（6.25 and 12.5 g/kg body weight）of DHA,and killed at 0,1,2,4,8,16 and 24 h. Total RNA was isolated from adipose tissue and liver. SQ （semi-quantitative） RT-PCR was used to analyze temporal expression of PPAR）＇, SREBP-1c, FAS, HSL and TGH. [Result] Data showed that DHA up-regulated the expression of PPAR）＇,FAS,HSL and TGH in adipose,whose expression reached the highest level at 8 h, and then descented. However,the expression of SREBP-1c was downregulated,reached the lowest level at 8 h, and then was upregulated by DHA. In addition, DHA down-regulated the expression of PPAR）,, SREBP-1c,FAS and HSL in liver,whose expression achieved the lowest level at 8,8,8 and 16 h respec- tively, and then increased. Furthermore there was no significant change for TGH gene （P〉0.05）.Concousion] It indicates that DHA regulated adipose deposition by increasing lipolytic genes in adipose tissue and inhibiting lipogenic and lipolytic genes in liver.

关 键 词：DHA 小鼠 脂肪组织 肝脏 生脂基因 脂解基因 

分 类 号：R151.2[医药卫生—营养与食品卫生学]