康复训练对脑梗死大鼠梗死灶周围Nogo—A表达的影响  

The effects of rehabilitation training on the expression of Nogo-A in the area surrounding cerebral infarction

作　　者：段淑荣[1] 孙林琳[1] 杨昆鹏[2] 王春燕[1] 沈月娥[1] 王德生[1] 张黎明[1] 

机构地区：[1]哈尔滨医科大学附属第一医院神经内科,哈尔滨150001 [2]哈尔滨医科大学附属第一医院血液内科,哈尔滨150001

出　　处：《中华物理医学与康复杂志》2008年第6期370-373,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：黑龙江省自然科学基金资助项目（D2004-07）

摘　　要：目的研究康复训练对大鼠脑梗死灶周围白质Nogo—A表达的影响。方法采用完全随机化方法将60只Wistar大鼠分成康复训练组和造模对照组，每组30只，应用Longa颈外动脉线栓法制备大鼠大脑中动脉闭塞模型。康复训练组大鼠每天进行滚筒、平衡木、转棒及网屏训练，时间共1h；造模对照组置于普通笼中自由活动。每组分别于造模后3，7，14，21和28d5个时间点随机选取6只大鼠进行行为学评估后处死，取脑组织采用免疫组织化学方法观察每个时间点脑梗死灶周围白质Nogo—A的表达。结果①康复训练组术后14，21和28d行为学评分明显低于造模对照组，差异具有统计学意义（P〈0．05）。②2组大鼠Nogo—A阳性细胞于脑缺血7d后开始增加，21d达高峰；脑缺血14d，21d和28d时间点。康复训练组Nogo—A阳性细胞的表达水平明显低于造模对照组，差异具有统计学意义（P〈0．05）。结论康复训练能通过减少脑梗死大鼠梗死灶周围Nogo—A的表达，减低其对神经轴突生长抑制作用，从而促进脑梗死后神经功能的恢复。Objective To study the effects of rehabilitation training on the expression of Nogo-A around the area of cerebral infraction using rats. Methods A total of 60 Wistar rats were randomly divided into a rehabilitation group and a control group after an experimental cerehral infarction had been established in them. The animals in the rehabilitation group were given exercise with a rotating bar, a balance beam and a rolling cage for one hour daily, while those in the control group were caged without any abnormal exercise. Nogo-A expression in the area surrounding the infarcts was detected by imunohistochemical techniques at the 3rd, 7th, 14th, 21st and 28th day after infarction. Meanwhile, neurobehavioral evaluations were also conducted. Results The animals in the rehabilitation group scored much lower than the controls in the behavioral evaluations at the 14th, 21st and 28th day. The expression of Nogo-A in tissues around the infracted area increased by the 7th day and peaked at the 21st day in both groups, but the expression of Nogo-A was significantly stronger in the rehabilitation group at the 14th, 21st and 28th days. Conclusion Rehabilitation training decreased the expression of Nogo-A in the brain of rats after infarction. This may have important implications for the functional recovery of the central nervous system.

关 键 词：脑梗死 康复训练 Nogo—A 神经再生 

分 类 号：R743.3[医药卫生—神经病学与精神病学] R392.11[医药卫生—临床医学]