干扰素-γ对大鼠肝组织中的MMP-13与TIMP-1表达的干预作用  

Effects of Interferon-γ on the Expression of Matrix Metalloproteinase-13 and Tissue Inhibitor of Metalloproteinase-1 in the Livers of Rats with Fibrosis

作　　者：陈莎燕[1] 曾令兰[1] 王华[1] 李淑莉[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院感染科实验室,武汉430022

出　　处：《临床消化病杂志》2008年第3期136-139,共4页Chinese Journal of Clinical Gastroenterology

摘　　要：目的探讨干扰素-γ对实验性大鼠肝组织中的基质金属蛋白酶-13(MMP-13)与金属蛋白酶组织抑制因子-1(TIMP-1)表达的影响。方法SD大鼠22只,随机分为3组:正常组(7只)、模型组(6只)和干扰素-γ组(9只);用四氯化碳制备大鼠肝纤维化模型,在10周末处死各组大鼠,取肝脏进行HE染色,并运用半定量逆转录聚合酶链反应(RT-PCR)检测肝组织中MMP-13 mRNA与TIMP-1 mRNA的表达量。结果MMP-13 mRNA的表达,与正常组对比,模型组和干扰素-γ组的MMP-13 mRNA的表达量增高(P<0.01),但模型组和干扰素-γ组之间还不能认为有统计学差别(P>0.01);而TIMP-1 mRNA表达与正常组相比,模型组和干扰素-γ组中的TIMP-1 mRNA的表达都升高(P<0.01),而且模型组中的TIMP-1 mRNA表达量比干扰素-γ组高(P<0.01)。在肝纤维化病理学观察的量化秩和分析中,各组之间的差别明显(P<0.005)。结论干扰素-γ逆转肝纤维化的机制可能是减少肝纤维化大鼠肝脏中的TIMP-1mR-NA的表达,从而逆转肝纤维化。Objective To investigate the effects of interferon-γ extract on the expression of the matrix metallo proteinase- 13 （ MMP-13 ） and the tisse inhibitor of metalloproteinase-1 （ TIMP-1 ） in hepatic tissue in experimental rats. Methods 22 male Sprague-Dawley rats were randomly divided into 3 groups：normal group（ 7 ） , model group（ 6 ） and interferon-γ group （9）. Hepatic fibrosis was induced by CCl4. All group of rats were sacrificed at the end of the 10th weeks,HE staining of hepatic tissue was performed,the semi-quantitative polymerase chain reaction （RT-PCR） assayed the mRNA expression of MMP-13 and TIMP- 1. Results The mRNA expression of MMP-13 among all groups were not all the same（ P 〈0.01 ） ,the model group and the interferon-γ group had the mRNA expression of MMP-13 more than the normal group（ P 〈0.01 ） ,but it could not be assumed sta- titiscally different between the model group and the interferon-γ group（ P 〉 0.01 ）. On the contrary, the mRNA expression of TIMP-1 had the remarkable difference among all groups（ P 〈0. 01 ） ,Compared with the normal group,the model group and the interferon-γ group obviously elevated（ P 〈0. 001 ） ,and TIMP-1 mRNA of the model group was more than that of the interferon- γ group（ P 〈 0.01 ）. There were great differences among these groups with rank test in the pathologic observation of liver fibrosis （ P 〈 0. 005 ）. Conclusion The mechanism of interferon-γ reversing liver fibrosis possibly maybe reduce the mRNA expression of TIMP-1, restore the liver collagen degeneration system gradually, thus reverse liver fibrosis.

关 键 词：肝纤维化 干扰素-Γ 基质金属蛋白酶-13 金属蛋白酶抑制因子-1 

分 类 号：R575.2[医药卫生—消化系统]