rassfia和survivin在胃癌中的表达及相互作用  

Expression and Correlation of Rassfia and Survivin in Gastric Carcinoma

作　　者：蔡兆根[1] 于东红[1] 章成兵[2] 冯振中[1] 汪向明[3] 

机构地区：[1]蚌埠医学院病理学教研室 [2]蚌埠医学院附属第二医院消化内科 [3]皖南医学院附属弋矶山医院病理科,蚌埠233003

出　　处：《临床消化病杂志》2008年第3期153-156,共4页Chinese Journal of Clinical Gastroenterology

摘　　要：目的检测胃癌组织、癌旁正常组织中rassfia基因和survivin基因的表达情况,探讨两者在胃癌发生、发展中的作用及其相互关系。方法用逆转录聚合酶链式反应(RT-PCR)方法检测49例胃癌组织、20例癌旁正常组织中的rassfia和survivin mRNA的表达情况。结果rassfia在胃癌组织中的转录表达缺失率为51%(25/49),在正常组织中完全表达,其表达缺失率与胃癌分化程度、淋巴结转移与否及TNM分期相关(P<0.05),但与其组织学类型无相关性。survivin在胃癌组织中转录表达率为65.3%(32/49),在正常组织中无阳性表达,其表达与胃癌分化程度、淋巴结转移与否相关(P<0.05),但与其TNM分期和组织学类型无相关性。结论rassfia基因是胃癌的相关抑癌基因,在胃癌中存在表达缺失;survivin作为癌基因在胃癌组织中表达上调。rassfia的表达缺失和survivn的表达上调具有相关性,两者共同参与了胃癌的发生和发展。Objective To explore the expression and correlation of rassfia and survivin in human primary gastric carcinoma. Methods RT-PCR was used to measure the mRNA expression of rassfia and survivin in 49 cases of primary gastric carcinoma,20 matched normal tissues. Results Expression loss of rassfia in 49 primary gastric carcinomas were 51% （25/49）, RASSF1A mRNA expression loss was positively related with histological differentiation degree（ P 〈 0.05 ） ,lymph node metastasis（ P 〈0. 05） and TNM stages（ P 〈0.05）. The expression rate of survivin mRNA was 65.3% （32/49） ,survivin mRNA expression was positively related with histological differentiation degree（ P 〈 0. 05 ） ,lymph node metastasis （ P 〈 0. 05 ） . Conclusion rassfia gene is a related tumor suppressor gene of gastric carcinoma, its expresstion is lost in gastric cancer. Survivin expression raises in gastric carcinoma. There is reverse relationship between rassfia expression lost and survivin expression, which participate commonly in gastric tumorigenesis and development.

关 键 词：胃肿瘤 rassfia基因 suvivin基因 RT—PCR 

分 类 号：R735.2[医药卫生—肿瘤]