HIF-1α、COX-2在溃疡性结肠炎中的表达及与疾病活动性的关系  

Expression of Hypoxia-inducible Factor-l alpha (HIF-1α) and Cyclooxygenase-2 (COX-2) in Ulcerative Colitis and Their Relationships with Activity of Ulecrative Colitis

作　　者：刘波 许春梅 

机构地区：[1]湖北省襄樊市第一人民医院消化内科,襄樊441000

出　　处：《临床消化病杂志》2008年第3期172-175,共4页Chinese Journal of Clinical Gastroenterology

基　　金：武汉市科技局资助项目(NO:301121017)

摘　　要：目的探讨低氧诱导因子-1α(HIF-1α)、环氧合酶-2(COX-2)在溃疡性结肠炎组织中的表达及与疾病活动性的关系。方法采用免疫组织化学方法检测51例UC患者(活动期38例,缓解期13例)和20例正常对照组中的HIF-1α、COX-2表达。结果溃疡性结肠炎患者活动期HIF-1α表达显著高于正常人,差异有显著性(153.29±15.26vs.193.28±16.65)(P<0.01),缓解期HIF-1α表达与正常人差异无显著性(185.45±12.08vs.193.28±16.65)(P>0.05)。溃疡性结肠炎中HIF-1α的表达与Walmsley评分标准有相关性,且呈正相关;COX-2在溃疡性结肠炎患者的活动期的表达显著高于正常人,差异有显著性(156.45±18.25vs.199.28±15.43)(P<0.01),缓解期的表达与正常人差异无显著性(190.93±13.78vs.199.28±15.43)(P>0.05)。溃疡性结肠炎中COX-2的表达与Walmsley评分标准也呈正相关;溃疡性结肠炎中HIF-1α和COX-2蛋白表达水平呈正相关(r=0.690)(P<0.5)。结论HIF-1α和COX-2均参与了溃疡性结肠炎的发病且与疾病的活动性有关,HIF-1α作为一种转录因子可能是COX-2均参与了溃疡性结肠炎的发病且与疾病的活动性有关,HIF-1α作为一种转录因子可能是COX-2基因表达中的一个重要调节者。Objective To investigate the expression of HIF-1 α and COX-2 in the tissues of patients with ulcerative colitis （UC） and their relationships with activity of UC. Methods Immunohistochemistry assay was performed to detect the expression of HIF-1α and COX-2 in the samples from 38 cases with UC in active stage, 13 cases at stable,and 20 cases in normal control. Results The expression of HIF-1 α was higher in patients with UC at active stage than that in the controls（ 153.29 ± 15.26 vs. 193.28 ± 16.65 ）, （ P 〈0. 1 ） ,while the expression of HIF-1α in UC at stable stage was not significantly different from that in the controls（ 185.45 ± 12. 08 vs. 193.28 ± 16.65 ） , （ P 〉 0. 05 ）. The level of HIF-1 α in the tissues in active UC was positively correlated with the Walmsley renal scoring（ r = 0. 531, P = 0. 001 ）. The expression of COX-2 was higher in patients with UC at active stage than that in the controls（ 156.45 ± 18. 25 vs. 199. 28 ± 15.43）, （ P 〈0. 01 ） ,while the expression of COX-2 in UC at stable stage was not significantly different from that in the controls（ 190. 93± 13. 78 vs. 199. 28 ± 15.43 ）（ P 〉 0. 05）. The level of COX-2 in the tissues in active UC was positively correlated with the Walmsley renal scoring（ r =0. 625, P 〈0. 01 ）. L- inear correlation analysis showed that COX-2 protein was positively correlated with HIF-1 α（ r = 0. 690, P 〈 0. 05 ）. Conclusion HIF-1 α and COX-2 are involved in the pathogenesis of UC, HIF-1α as a transcriptional factor may be an essential regulator of COX-2 gene expression.

关 键 词：溃疡性结肠炎 低氧诱导因子-1Α 环氧合酶-2 免疫组化 

分 类 号：R574.6[医药卫生—消化系统]