N-去硫酸肝素对体外培养人胃癌SGC-7901细胞bFGF表达的影响  

In vitro effect of N-desulfated heparin on the expression of basic fibroblast growth factor in gastric carcinoma cell line SGC-7901

作　　者：陈明祥 陈金联 陆金来 洪静 陈维雄 朱金水 陈尼维 耿建国[2] 

机构地区：[1]上海市第六人民医消化内科,上海市200233 [2]中国科学院上海细胞生物学研究所,上海市200031

出　　处：《世界华人消化杂志》2008年第17期1920-1925,共6页World Chinese Journal of Digestology

摘　　要：目的:探讨N-去硫酸肝素对体外培养人胃癌SGC-7901细胞碱性成纤维细胞生长因子(bFGF)表达的影响.方法:体外培养人胃癌SGC-7901细胞,加入含不同浓度N-去硫酸肝素(0.1、1.0g/L,N-去硫酸肝素组)的RPMI 1640培养液,并设对照(为培养液),每组平行3个样本.培养12、24h,应用双抗体夹心ABC-ELISA法及实时荧光定量PCR法检测胃癌细胞bFGF的表达.结果:与对照组相比,0.1、1.0g/LN-去硫酸肝素组培养12、24h,bFGF的表达下降均有统计学意义(t=7.502,P=0.002;t=55.416,P=0.000;t=52.221,P=0.000;t=48.080,P=0.000);相同时间下各浓度的N-去硫酸肝素组中bFGFmRNA的表达(CT值)较对照组高.N-去硫酸肝素对胃癌细胞bFGF蛋白及mRNA表达的抑制作用具有剂量及时间依赖性.结论:N-去硫酸肝素可以显著抑制体外培养人胃癌细胞bFGF的表达,且具有时间、剂量依赖性.AIM： To investigate the effect of N-desulfated heparin on the expression of basic fibroblast growth factor （bFGF） in human gastric carcinoma SGC-7901 cells in vitro. METHODS： Human gastric carcinoma cell line SGC-7901 was cultured in vitro, and then treated with N-desulfated heparin at different concentrations （0.1, 1.0 g/L）. Meanwhile, SGC-7901 cells cultured in single RPMI 1640 medium were used as controls. Each group contained 3 paralleled samples. Enzyme-linked immunosorbent assay （ELISA） and real time polymerase chain reaction （PCR） were used to detect the expression of bFGF in SGC-7901 cells at 12 h and 24 h, respectively. RESULTS： After treatment with 0.1 or 1.0 g/L N-desulfated heparin for 12 or 24 h, bFGF protein expression was decreased significantly （t = 7.502, P = 0.002; t = 55.416, P = 0.000; t = 52.221, P = 0.000; t = 48.080, P = 0.000）. The expression of bFGF （CT value） in each N-desulfated heparin group was higher than that in the control group at the same time. The inhibition of N-desulfated heparin on bFGF protein and mRNA expression in SGC-7901 cells was dose- and time-dependent. CONCLUSION： N-desulfated heparin can inhibit the expression of bFGF in gastric cancer cell line SGC-7901 in a dose- and time-dependent manner in vitro.

关 键 词：N-去硫酸肝素 SGC-7901胃癌细胞 碱性成纤维细胞生长因子 ELISA 实时荧光定量PCR 

分 类 号：R735.2[医药卫生—肿瘤] R692[医药卫生—临床医学]