早、中、晚孕期胎盘因子对人外周血淋巴细胞CD4,CCR5和CXCR4表达的影响(英文)  

Effect of first, second, and third trimester placental factors on CD4, CCR5,and CXCR4 expression in human peripheral blood lymphocytes

作　　者：李莉平[1] 康佳丽[1] 夏薇[1] 曾耀英[2] 

机构地区：[1]广州市第一人民医院妇产科,广州510180 [2]暨南大学组织移植与免疫中心,广州510632

出　　处：《中南大学学报（医学版）》2008年第6期461-467,共7页Journal of Central South University ：Medical Science

基　　金：This work was supported by the National Natural Science Foundation of China(30500466)

摘　　要：目的:通过研究早、中、晚孕期胎盘因子(PF)对人外周血淋巴细胞(PBLs)中CD4,CCR5和CXCR4表达的作用,探讨PF在人免疫缺陷病毒-1(HIV-1)垂直传播中的作用及其机制。方法:制备早、中、晚孕期PF。分离人外周血单个核细胞,并分别与相对浓度为25%的早、中、晚孕期PF作用,培养24h后收集细胞,荧光抗体标记,流式细胞术检测外周血淋巴细胞(PBLs)中CD4,CCR5和CXCR4表达,以及CD4+T细胞中CCR5+细胞、CXCR4+细胞、CCR5+CXCR4+细胞所占的百分率。结果:各孕期PF均可显著降低PBLs中CCR5的表达,其中早孕期PF的作用明显强于中、晚孕期PF的作用;各孕期PF组CD4+T细胞中CCR5+细胞的百分率均显著低于对照组,早孕期PF组CD4+T细胞中CCR5+细胞的百分率明显低于中、晚孕期PF组;各孕期PF组CD4+T细胞中CCR5+CXCR4+细胞的百分率均显著低于对照组,早孕期PF组CD4+T细胞中CCR5+CXCR4+细胞的百分率显著低于晚孕期PF组。结论:各孕期PF均可显著降低PBLs中CCR5的表达,以及CD4+T细胞中CCR5+细胞和CCR5+CXCR4+细胞的百分率,早孕期PF作用最强,中、晚孕期PF效应相当,PF可能通过抑制R5病毒的入胞而具有抗R5病毒的作用,并可能在阻断HIV-1宫内感染中具有重要作用。Objective To investigate effect of first, second, and third trimester placental factors （PF） on CD4, CCR5, and CXCR4 expression in human peripheral blood lymphocytes （PBLs）, and to explore their influence on human immunodeficiency virus （HIV） vertical transmission.Methods Human peripheral blood mononuclear cells （PBMCs） were treated with first, second,and third trimester PF （concentration 25%） respectively for 24 hours. The expression of CD4, CCR5,and CXCR4 in PBLs, and the percentages of CCR5^＋, CXCR4^＋,and CCR5^＋CXCR4^＋ cells in peripheral blood CD4^＋ lymphocytes were determined with flow cytometry.Results All trimester PFs reduced CCR5 expression in PBLs. The efficiency of the first trimester PF was higher than that of the second and third trimester PF. The percentage of CCR5^＋ cells in peripheral blood CD4^＋ lymphocytes of PF groups was significantly lower than that of the control group, and the percentage of CCR5^＋ cells in peripheral blood CD4^＋ lymphocytes of the first trimester PF group was significantly lower than that of the second and third trimester group. The percentages of CCR5^＋CXCR4^＋ cells in peripheral blood CD4^＋ lymphocytes of PF groups were significantly decreased as compared with the control group, and the percentage of CCR5^＋CXCR4^＋ cells in peripheral blood CD4^＋ lymphocytes of the first trimester PF group was significantly lower than that of the third trimester PF group.Conclusion PF can reduce the expression of CCR5 in human PBLs and peripheral blood CD4^＋ lymphocytes, indicating that PF might reduce R5 virus infection via preventing HIV entry, and might play an important role in reducing R5 virus intrauterine infection.

关 键 词：胎盘因子 HIV-1 垂直传播 CD4 CCR5 CXCR4 

分 类 号：R714[医药卫生—妇产科学]